TY  - JOUR
AU  - Reis, Vinicius Pinho Dos
AU  - Keller, Markus
AU  - Schmidt, Katja
AU  - Ulrich, Rainer Günter
AU  - Groschup, Martin Hermann
TI  - αVβ3 Integrin Expression Is Essential for Replication of Mosquito and Tick-Borne Flaviviruses in Murine Fibroblast Cells.
JO  - Viruses
VL  - 14
IS  - 1
SN  - 1999-4915
CY  - Basel
PB  - MDPI
M1  - DKFZ-2022-00154
SP  - 18
PY  - 2022
AB  - The Flavivirus genus includes a number of important viruses that are pathogenic to humans and animals and are responsible for outbreaks across the globe. Integrins, a family of heterodimeric transmembrane molecules expressed in all nucleated cells mediate critical functions of cell physiology and cell cycle. Integrins were previously postulated to be involved in flavivirus entry and to modulate flavivirus replication efficiency. In the present study, mouse embryonic fibroblasts (MEF), lacking the expression of αVβ3 integrin (MEF-αVβ3-/-), were infected with four different flaviviruses, namely yellow fever virus (YFV), West Nile virus (WNV), Usutu virus (USUV) and Langat virus (LGTV). The effects of the αVβ3 integrin absence in double-knockout MEF-αVβ3-/- on flavivirus binding, internalization and replication were compared to the respective wild-type cells. Binding to the cell surface for all four flaviviruses was not affected by the ablation of αVβ3 integrin, whereas internalization of USUV and WNV was slightly affected by the loss of αVβ3 integrin expression. Most interestingly, the deletion of αVβ3 integrin strongly impaired replication of all flaviviruses with a reduction of up to 99
KW  - flavivirus (Other)
KW  - host cell factors (Other)
KW  - integrins (Other)
KW  - virus binding (Other)
KW  - virus internalization (Other)
KW  - virus replication (Other)
LB  - PUB:(DE-HGF)16
C6  - pmid:35062222
DO  - DOI:10.3390/v14010018
UR  - https://inrepo02.dkfz.de/record/178605
ER  -