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@ARTICLE{Bankoglu:178826,
      author       = {E. E. Bankoglu and T. Mukama$^*$ and V. Katzke$^*$ and F.
                      Stipp and T. Johnson$^*$ and T. Kühn$^*$ and F. Seyfried
                      and R. Godschalk and A. Collins and R. Kaaks$^*$ and H.
                      Stopper},
      title        = {{S}hort- and long-term reproducibility of the {COMET} assay
                      for measuring {DNA} damage biomarkers in frozen blood
                      samples of the {EPIC}-{H}eidelberg cohort.},
      journal      = {Mutation research / Genetic toxicology and environmental
                      mutagenesis},
      volume       = {874-875},
      issn         = {1383-5718},
      address      = {Amsterdam [u.a.]},
      publisher    = {Elsevier Science},
      reportid     = {DKFZ-2022-00290},
      pages        = {503442},
      year         = {2022},
      abstract     = {The comet assay is widely used for quantification of
                      genomic damage in humans. Peripheral blood derived
                      mononuclear cells (PBMCs) are the most often used cell type
                      for this purpose. Since the comet assay can be performed in
                      an enhanced throughput format, it can be applied to large
                      sample collections such as biobanks. The European
                      Prospective Investigation into Cancer and Nutrition (EPIC)
                      study is one of the largest existing prospective cohort
                      studies, and the German Cancer Research Institute (DKFZ) in
                      Heidelberg is a participating center with 25.000 frozen
                      blood samples stored from around 25 years ago, enabling
                      retrospective assessment of disease risk factors. However,
                      experience with decades long frozen samples in the comet
                      assay is so far missing. In Heidelberg, 800 study
                      participants were re-invited twice between 2010 and 2012 to
                      donate further blood samples. Here, we analyzed 299
                      Heidelberg-EPIC samples, compiled from frozen PBMC and buffy
                      coat preparations selected from the different sampling time
                      points. In addition, 47 frozen PBMC samples from morbidly
                      obese individuals were included. For buffy coat samples, we
                      observed a poor correlation between DNA damage in the same
                      donors assessed at two sampling time points. Additionally,
                      no correlation between DNA damage in buffy coat samples and
                      PBMCs was found. For PBMCs, a good correlation was observed
                      between samples of the same donors at the two time points.
                      DNA damage was not affected by age and smoking status, but
                      high BMI (>30; obesity) was associated with increased DNA
                      damage in PBMCs. There was no indication for a threshold of
                      a certain BMI for increased DNA damage. In conclusion, while
                      25 year-long stored buffy coat preparations may require
                      adaptation of certain experimental parameters such as cell
                      density and electrophoresis conditions, frozen PBMC biobank
                      samples can be analyzed in the comet assay even after a
                      decade of storage.},
      keywords     = {Biobank (Other) / Comet assay (Other) / DNA damage (Other)
                      / EPIC (Other) / Human biomonitoring (Other)},
      cin          = {C020},
      ddc          = {570},
      cid          = {I:(DE-He78)C020-20160331},
      pnm          = {313 - Krebsrisikofaktoren und Prävention (POF4-313)},
      pid          = {G:(DE-HGF)POF4-313},
      typ          = {PUB:(DE-HGF)16},
      pubmed       = {pmid:35151425},
      doi          = {10.1016/j.mrgentox.2022.503442},
      url          = {https://inrepo02.dkfz.de/record/178826},
}