000178894 001__ 178894
000178894 005__ 20240229143558.0
000178894 0247_ $$2doi$$a10.1093/nar/gkac113
000178894 0247_ $$2pmid$$apmid:35188570
000178894 0247_ $$2ISSN$$a0305-1048
000178894 0247_ $$2ISSN$$a0309-1872
000178894 0247_ $$2ISSN$$a1362-4954
000178894 0247_ $$2ISSN$$a1362-4962
000178894 0247_ $$2altmetric$$aaltmetric:123447961
000178894 037__ $$aDKFZ-2022-00325
000178894 041__ $$aEnglish
000178894 082__ $$a570
000178894 1001_ $$0P:(DE-He78)ff9387efc3c4f931e364486766584bda$$aFrank, Lukas$$b0$$eFirst author$$udkfz
000178894 245__ $$aALT-FISH quantifies alternative lengthening of telomeres activity by imaging of single-stranded repeats.
000178894 260__ $$aOxford$$bOxford Univ. Press$$c2022
000178894 3367_ $$2DRIVER$$aarticle
000178894 3367_ $$2DataCite$$aOutput Types/Journal article
000178894 3367_ $$0PUB:(DE-HGF)16$$2PUB:(DE-HGF)$$aJournal Article$$bjournal$$mjournal$$s1656420724_18200
000178894 3367_ $$2BibTeX$$aARTICLE
000178894 3367_ $$2ORCID$$aJOURNAL_ARTICLE
000178894 3367_ $$00$$2EndNote$$aJournal Article
000178894 500__ $$a#EA:B066#LA:B066# / 2022 Jun 24;50(11):e61
000178894 520__ $$aAlternative lengthening of telomeres (ALT) occurs in ∼10% of cancer entities. However, little is known about the heterogeneity of ALT activity since robust ALT detection assays with high-throughput in situ readouts are lacking. Here, we introduce ALT-FISH, a method to quantitate ALT activity in single cells from the accumulation of single-stranded telomeric DNA and RNA. It involves a one-step fluorescent in situ hybridization approach followed by fluorescence microscopy imaging. Our method reliably identified ALT in cancer cell lines from different tumor entities and was validated in three established models of ALT induction and suppression. Furthermore, we successfully applied ALT-FISH to spatially resolve ALT activity in primary tissue sections from leiomyosarcoma and neuroblastoma tumors. Thus, our assay provides insights into the heterogeneity of ALT tumors and is suited for high-throughput applications, which will facilitate screening for ALT-specific drugs.
000178894 536__ $$0G:(DE-HGF)POF4-312$$a312 - Funktionelle und strukturelle Genomforschung (POF4-312)$$cPOF4-312$$fPOF IV$$x0
000178894 588__ $$aDataset connected to CrossRef, PubMed, , Journals: inrepo01.inet.dkfz-heidelberg.de
000178894 7001_ $$0P:(DE-He78)0a1ab2219242b53553fbdce3aa73e9cf$$aRademacher, Anne$$b1$$udkfz
000178894 7001_ $$0P:(DE-He78)6a07580372bad8f82ab01df2ed28c545$$aMücke, Norbert$$b2$$udkfz
000178894 7001_ $$0P:(DE-He78)1670176f1912f8359289bb834eb62700$$aTirier, Stephan M$$b3$$udkfz
000178894 7001_ $$0P:(DE-He78)0ffda3563e61bab32f68ec2b4875d242$$aKoeleman, Emma$$b4$$udkfz
000178894 7001_ $$0P:(DE-He78)e4aeedd04506c1a7298cea5a5946bc7d$$aKnotz, Caroline$$b5$$udkfz
000178894 7001_ $$0P:(DE-He78)d70ddd73ff941566d830600443d2e69c$$aSchumacher, Sabrina$$b6$$udkfz
000178894 7001_ $$0P:(DE-He78)311fe8118b7563b455ba84e94fccb3a8$$aStainczyk, Sabine A$$b7$$udkfz
000178894 7001_ $$0P:(DE-He78)91f32735ee876c579d63c05a7f4778dd$$aWestermann, Frank$$b8$$udkfz
000178894 7001_ $$0P:(DE-He78)f0144d171d26dbedb67c9db1df35629d$$aFröhling, Stefan$$b9$$udkfz
000178894 7001_ $$0P:(DE-He78)97208bef945f0ab6bf33ed9925685de4$$aChudasama, Priya$$b10$$udkfz
000178894 7001_ $$0P:(DE-He78)94de5f7413279464b6e738d91dfae1eb$$aRippe, Karsten$$b11$$eLast author$$udkfz
000178894 773__ $$0PERI:(DE-600)1472175-2$$a10.1093/nar/gkac113$$gp. gkac113$$n11$$pe61$$tNucleic acids research$$v50$$x0301-5610$$y2022
000178894 909CO $$ooai:inrepo02.dkfz.de:178894$$pVDB
000178894 9101_ $$0I:(DE-588b)2036810-0$$6P:(DE-He78)ff9387efc3c4f931e364486766584bda$$aDeutsches Krebsforschungszentrum$$b0$$kDKFZ
000178894 9101_ $$0I:(DE-588b)2036810-0$$6P:(DE-He78)0a1ab2219242b53553fbdce3aa73e9cf$$aDeutsches Krebsforschungszentrum$$b1$$kDKFZ
000178894 9101_ $$0I:(DE-588b)2036810-0$$6P:(DE-He78)6a07580372bad8f82ab01df2ed28c545$$aDeutsches Krebsforschungszentrum$$b2$$kDKFZ
000178894 9101_ $$0I:(DE-588b)2036810-0$$6P:(DE-He78)1670176f1912f8359289bb834eb62700$$aDeutsches Krebsforschungszentrum$$b3$$kDKFZ
000178894 9101_ $$0I:(DE-588b)2036810-0$$6P:(DE-He78)0ffda3563e61bab32f68ec2b4875d242$$aDeutsches Krebsforschungszentrum$$b4$$kDKFZ
000178894 9101_ $$0I:(DE-588b)2036810-0$$6P:(DE-He78)e4aeedd04506c1a7298cea5a5946bc7d$$aDeutsches Krebsforschungszentrum$$b5$$kDKFZ
000178894 9101_ $$0I:(DE-588b)2036810-0$$6P:(DE-He78)d70ddd73ff941566d830600443d2e69c$$aDeutsches Krebsforschungszentrum$$b6$$kDKFZ
000178894 9101_ $$0I:(DE-588b)2036810-0$$6P:(DE-He78)311fe8118b7563b455ba84e94fccb3a8$$aDeutsches Krebsforschungszentrum$$b7$$kDKFZ
000178894 9101_ $$0I:(DE-588b)2036810-0$$6P:(DE-He78)91f32735ee876c579d63c05a7f4778dd$$aDeutsches Krebsforschungszentrum$$b8$$kDKFZ
000178894 9101_ $$0I:(DE-588b)2036810-0$$6P:(DE-He78)f0144d171d26dbedb67c9db1df35629d$$aDeutsches Krebsforschungszentrum$$b9$$kDKFZ
000178894 9101_ $$0I:(DE-588b)2036810-0$$6P:(DE-He78)97208bef945f0ab6bf33ed9925685de4$$aDeutsches Krebsforschungszentrum$$b10$$kDKFZ
000178894 9101_ $$0I:(DE-588b)2036810-0$$6P:(DE-He78)94de5f7413279464b6e738d91dfae1eb$$aDeutsches Krebsforschungszentrum$$b11$$kDKFZ
000178894 9131_ $$0G:(DE-HGF)POF4-312$$1G:(DE-HGF)POF4-310$$2G:(DE-HGF)POF4-300$$3G:(DE-HGF)POF4$$4G:(DE-HGF)POF$$aDE-HGF$$bGesundheit$$lKrebsforschung$$vFunktionelle und strukturelle Genomforschung$$x0
000178894 9141_ $$y2022
000178894 915__ $$0LIC:(DE-HGF)CCBYNCNV$$2V:(DE-HGF)$$aCreative Commons Attribution-NonCommercial CC BY-NC (No Version)$$bDOAJ$$d2021-05-04
000178894 915__ $$0StatID:(DE-HGF)0160$$2StatID$$aDBCoverage$$bEssential Science Indicators$$d2021-05-04
000178894 915__ $$0StatID:(DE-HGF)1190$$2StatID$$aDBCoverage$$bBiological Abstracts$$d2021-05-04
000178894 915__ $$0StatID:(DE-HGF)0113$$2StatID$$aWoS$$bScience Citation Index Expanded$$d2021-05-04
000178894 915__ $$0StatID:(DE-HGF)0561$$2StatID$$aArticle Processing Charges$$d2021-05-04
000178894 915__ $$0StatID:(DE-HGF)0561$$2StatID$$aArticle Processing Charges$$d2021-05-04
000178894 915__ $$0StatID:(DE-HGF)0700$$2StatID$$aFees$$d2021-05-04
000178894 915__ $$0StatID:(DE-HGF)0420$$2StatID$$aNationallizenz$$d2022-11-18$$wger
000178894 915__ $$0StatID:(DE-HGF)0100$$2StatID$$aJCR$$bNUCLEIC ACIDS RES : 2021$$d2022-11-18
000178894 915__ $$0StatID:(DE-HGF)0200$$2StatID$$aDBCoverage$$bSCOPUS$$d2022-11-18
000178894 915__ $$0StatID:(DE-HGF)0300$$2StatID$$aDBCoverage$$bMedline$$d2022-11-18
000178894 915__ $$0StatID:(DE-HGF)0501$$2StatID$$aDBCoverage$$bDOAJ Seal$$d2022-09-23T13:40:12Z
000178894 915__ $$0StatID:(DE-HGF)0500$$2StatID$$aDBCoverage$$bDOAJ$$d2022-09-23T13:40:12Z
000178894 915__ $$0StatID:(DE-HGF)0030$$2StatID$$aPeer Review$$bDOAJ : Blind peer review$$d2022-09-23T13:40:12Z
000178894 915__ $$0StatID:(DE-HGF)0199$$2StatID$$aDBCoverage$$bClarivate Analytics Master Journal List$$d2022-11-18
000178894 915__ $$0StatID:(DE-HGF)0150$$2StatID$$aDBCoverage$$bWeb of Science Core Collection$$d2022-11-18
000178894 915__ $$0StatID:(DE-HGF)1050$$2StatID$$aDBCoverage$$bBIOSIS Previews$$d2022-11-18
000178894 915__ $$0StatID:(DE-HGF)1030$$2StatID$$aDBCoverage$$bCurrent Contents - Life Sciences$$d2022-11-18
000178894 915__ $$0StatID:(DE-HGF)9915$$2StatID$$aIF >= 15$$bNUCLEIC ACIDS RES : 2021$$d2022-11-18
000178894 9202_ $$0I:(DE-He78)B066-20160331$$kB066$$lB066 Chromatin-Netzwerke$$x0
000178894 9201_ $$0I:(DE-He78)B066-20160331$$kB066$$lB066 Chromatin-Netzwerke$$x0
000178894 9201_ $$0I:(DE-He78)B087-20160331$$kB087$$lB087 Neuroblastom Genomik$$x1
000178894 9201_ $$0I:(DE-He78)B340-20160331$$kB340$$lTranslationale Medizinische Onkologie$$x2
000178894 9201_ $$0I:(DE-He78)HD01-20160331$$kHD01$$lDKTK HD zentral$$x3
000178894 9201_ $$0I:(DE-He78)B390-20160331$$kB390$$lPräzisions-Sarkomforschung$$x4
000178894 9200_ $$0I:(DE-He78)B066-20160331$$kB066$$lB066 Chromatin-Netzwerke$$x0
000178894 980__ $$ajournal
000178894 980__ $$aVDB
000178894 980__ $$aI:(DE-He78)B066-20160331
000178894 980__ $$aI:(DE-He78)B087-20160331
000178894 980__ $$aI:(DE-He78)B340-20160331
000178894 980__ $$aI:(DE-He78)HD01-20160331
000178894 980__ $$aI:(DE-He78)B390-20160331
000178894 980__ $$aUNRESTRICTED