TY  - JOUR
AU  - Fresnais, Margaux
AU  - Karabulut, Seda
AU  - Abou Zeed, Yasmin
AU  - Ungermann, Johannes
AU  - Benzel, Julia
AU  - Pajtler, Kristian W
AU  - Pfister, Stefan M
AU  - Haefeli, Walter E
AU  - Burhenne, Jürgen
AU  - Longuespée, Rémi
TI  - Important Requirements for the Selection of Internal Standards during the Development of Desorption/Ionization Assays for Drug Quantification in Biological Matrices-A Practical Example.
JO  - Molecules
VL  - 27
IS  - 3
SN  - 1420-3049
CY  - Basel
PB  - MDPI
M1  - DKFZ-2022-00334
SP  - 690
PY  - 2022
AB  - Desorption/ionization mass spectrometry (DI-MS) approaches allow for the rapid quantification of drugs in biological matrices using assays that can be validated according to regulatory guidelines. However, specific adaptations must be applied to create reliable quantification methods, depending on the approach and instrumentation used. In the present article, we demonstrate the importance of the molecular weight, the fragmentation pattern, and the purity of the internal standard for the development of matrix-assisted laser desorption/ionization (MALDI)-ion mobility (IM)-tandem MS and MS/MS methods. We present preliminary results of method development for the quantification of selinexor in microdialysis fluids with a stable isotopically labeled internal standard. In addition, we discuss the selection of internal standards for MALDI-MS assays using different instrumentations.
KW  - Biological Assay
KW  - Chromatography, Liquid: methods
KW  - Mass Spectrometry: methods
KW  - Molecular Weight
KW  - Pharmaceutical Preparations: chemistry
KW  - Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization: methods
KW  - Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization: standards
KW  - Tandem Mass Spectrometry: methods
KW  - biological matrices (Other)
KW  - desorption/ionization (Other)
KW  - drug (Other)
KW  - mass spectrometry (Other)
KW  - quantification (Other)
KW  - Pharmaceutical Preparations (NLM Chemicals)
LB  - PUB:(DE-HGF)16
C6  - pmid:35163955
C2  - pmc:PMC8838468
DO  - DOI:10.3390/molecules27030690
UR  - https://inrepo02.dkfz.de/record/178903
ER  -