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001     178903
005     20240303005037.0
024 7 _ |a 10.3390/molecules27030690
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037 _ _ |a DKFZ-2022-00334
041 _ _ |a English
082 _ _ |a 540
100 1 _ |a Fresnais, Margaux
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245 _ _ |a Important Requirements for the Selection of Internal Standards during the Development of Desorption/Ionization Assays for Drug Quantification in Biological Matrices-A Practical Example.
260 _ _ |a Basel
|c 2022
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520 _ _ |a Desorption/ionization mass spectrometry (DI-MS) approaches allow for the rapid quantification of drugs in biological matrices using assays that can be validated according to regulatory guidelines. However, specific adaptations must be applied to create reliable quantification methods, depending on the approach and instrumentation used. In the present article, we demonstrate the importance of the molecular weight, the fragmentation pattern, and the purity of the internal standard for the development of matrix-assisted laser desorption/ionization (MALDI)-ion mobility (IM)-tandem MS and MS/MS methods. We present preliminary results of method development for the quantification of selinexor in microdialysis fluids with a stable isotopically labeled internal standard. In addition, we discuss the selection of internal standards for MALDI-MS assays using different instrumentations.
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650 _ 7 |a biological matrices
|2 Other
650 _ 7 |a desorption/ionization
|2 Other
650 _ 7 |a drug
|2 Other
650 _ 7 |a mass spectrometry
|2 Other
650 _ 7 |a quantification
|2 Other
650 _ 7 |a Pharmaceutical Preparations
|2 NLM Chemicals
650 _ 2 |a Biological Assay
|2 MeSH
650 _ 2 |a Chromatography, Liquid: methods
|2 MeSH
650 _ 2 |a Mass Spectrometry: methods
|2 MeSH
650 _ 2 |a Molecular Weight
|2 MeSH
650 _ 2 |a Pharmaceutical Preparations: chemistry
|2 MeSH
650 _ 2 |a Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization: methods
|2 MeSH
650 _ 2 |a Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization: standards
|2 MeSH
650 _ 2 |a Tandem Mass Spectrometry: methods
|2 MeSH
700 1 _ |a Karabulut, Seda
|b 1
700 1 _ |a Abou Zeed, Yasmin
|b 2
700 1 _ |a Ungermann, Johannes
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700 1 _ |a Benzel, Julia
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700 1 _ |a Pajtler, Kristian W
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700 1 _ |a Pfister, Stefan M
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700 1 _ |a Haefeli, Walter E
|0 0000-0003-0672-6876
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700 1 _ |a Burhenne, Jürgen
|0 0000-0002-2190-1698
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700 1 _ |a Longuespée, Rémi
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773 _ _ |a 10.3390/molecules27030690
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