000178967 001__ 178967
000178967 005__ 20240229143601.0
000178967 0247_ $$2doi$$a10.3390/cancers14030849
000178967 0247_ $$2pmid$$apmid:35159116
000178967 0247_ $$2pmc$$apmc:PMC8834194
000178967 0247_ $$2altmetric$$aaltmetric:123026199
000178967 037__ $$aDKFZ-2022-00383
000178967 041__ $$aEnglish
000178967 082__ $$a610
000178967 1001_ $$0P:(DE-He78)35c44ba0995477242f21a01ca69d6945$$aGatzweiler, Charlotte$$b0$$eFirst author$$udkfz
000178967 245__ $$aFunctional Therapeutic Target Validation Using Pediatric Zebrafish Xenograft Models.
000178967 260__ $$aBasel$$bMDPI$$c2022
000178967 3367_ $$2DRIVER$$aarticle
000178967 3367_ $$2DataCite$$aOutput Types/Journal article
000178967 3367_ $$0PUB:(DE-HGF)16$$2PUB:(DE-HGF)$$aJournal Article$$bjournal$$mjournal$$s1646126993_20517
000178967 3367_ $$2BibTeX$$aARTICLE
000178967 3367_ $$2ORCID$$aJOURNAL_ARTICLE
000178967 3367_ $$00$$2EndNote$$aJournal Article
000178967 500__ $$a#EA:B310#LA:B310#
000178967 520__ $$aThe survival rate among children with relapsed tumors remains poor, due to tumor heterogeneity, lack of directly actionable tumor drivers and multidrug resistance. Novel personalized medicine approaches tailored to each tumor are urgently needed to improve cancer treatment. Current pediatric precision oncology platforms, such as the INFORM (INdividualized Therapy FOr Relapsed Malignancies in Childhood) study, reveal that molecular profiling of tumor tissue identifies targets associated with clinical benefit in a subgroup of patients only and should be complemented with functional drug testing. In such an approach, patient-derived tumor cells are exposed to a library of approved oncological drugs in a physiological setting, e.g., in the form of animal avatars injected with patient tumor cells. We used molecularly fully characterized tumor samples from the INFORM study to compare drug screen results of individual patient-derived cell models in functional assays: (i) patient-derived spheroid cultures within a few days after tumor dissociation; (ii) tumor cells reisolated from the corresponding mouse PDX; (iii) corresponding long-term organoid-like cultures and (iv) drug evaluation with the corresponding zebrafish PDX (zPDX) model. Each model had its advantage and complemented the others for drug hit and drug combination selection. Our results provide evidence that in vivo zPDX drug screening is a promising add-on to current functional drug screening in precision medicine platforms.
000178967 536__ $$0G:(DE-HGF)POF4-312$$a312 - Funktionelle und strukturelle Genomforschung (POF4-312)$$cPOF4-312$$fPOF IV$$x0
000178967 588__ $$aDataset connected to CrossRef, PubMed, , Journals: inrepo01.inet.dkfz-heidelberg.de
000178967 650_7 $$2Other$$adrug screen
000178967 650_7 $$2Other$$afunctional precision oncology
000178967 650_7 $$2Other$$amPDX
000178967 650_7 $$2Other$$apatient-derived spheroid culture
000178967 650_7 $$2Other$$asmall molecule inhibitors
000178967 650_7 $$2Other$$atargeted therapy
000178967 650_7 $$2Other$$azPDX
000178967 7001_ $$0P:(DE-He78)53112ef656923758316e7079710bc988$$aRidinger, Johannes$$b1$$udkfz
000178967 7001_ $$0P:(DE-He78)ea170691ed65b95f10820c4c61d6e2cd$$aHerter, Sonja$$b2$$udkfz
000178967 7001_ $$0P:(DE-He78)4302629f88822e785a2ba95b985cc952$$aGerloff, Xenia F$$b3$$udkfz
000178967 7001_ $$0P:(DE-He78)2bebef4e65bbcb341e31ca0999b69436$$aElHarouni, Dina$$b4$$udkfz
000178967 7001_ $$0P:(DE-He78)b9b209c7129285852f61f807f332725a$$aBerker, Yannick$$b5$$udkfz
000178967 7001_ $$0P:(DE-He78)3c021852cf94cb29825e65baa82ed54b$$aImle, Roland$$b6$$udkfz
000178967 7001_ $$0P:(DE-He78)5ebf27c73a3f0b66abe1c471997cebb8$$aSchmitt, Lukas$$b7$$udkfz
000178967 7001_ $$0P:(DE-He78)21d657d65d4ad0fd8b0bbfe10fa65172$$aKreth, Sina$$b8$$udkfz
000178967 7001_ $$0P:(DE-He78)311fe8118b7563b455ba84e94fccb3a8$$aStainczyk, Sabine$$b9$$udkfz
000178967 7001_ $$0P:(DE-He78)32e22d1c8416af3f2a612da9121f2c05$$aAyhan, Simay$$b10$$udkfz
000178967 7001_ $$0P:(DE-He78)f08873535c440e3ce033c84d5786f70f$$aNajafi, Sara$$b11$$udkfz
000178967 7001_ $$0P:(DE-He78)2214defe87d04a849413e5eed47c415e$$aKrunic, Damir$$b12$$udkfz
000178967 7001_ $$aFrese, Karen$$b13
000178967 7001_ $$aMeder, Benjamin$$b14
000178967 7001_ $$0P:(DE-He78)d5149ffd74f42a2fa87a086d66645aaa$$aReuss, David$$b15$$udkfz
000178967 7001_ $$0P:(DE-He78)8de7015af76bd61fb0bc13bb3facb1df$$aFiesel, Petra$$b16$$udkfz
000178967 7001_ $$0P:(DE-He78)2f592d9d8339bee07cca3956b7472b61$$aSchramm, Kathrin$$b17$$udkfz
000178967 7001_ $$0P:(DE-He78)261d2b84aded878003e6e35d18113831$$aBlattner-Johnson, Mirjam$$b18$$udkfz
000178967 7001_ $$0P:(DE-He78)551bb92841f634070997aa168d818492$$aJones, David T W$$b19$$udkfz
000178967 7001_ $$0P:(DE-He78)990de6ec6c2f0a40b17ed75162ca19e8$$aBanito, Ana$$b20$$udkfz
000178967 7001_ $$0P:(DE-He78)91f32735ee876c579d63c05a7f4778dd$$aWestermann, Frank$$b21$$udkfz
000178967 7001_ $$0P:(DE-He78)55dfd5b374ab9bccdb49e06e91345571$$aOppermann, Sina$$b22$$udkfz
000178967 7001_ $$0P:(DE-He78)0be2f86573954f87e97f8a4dbb05cb0f$$aMilde, Till$$b23$$udkfz
000178967 7001_ $$0P:(DE-He78)2727b5cb63b52d0137d4f4e8f110ee7e$$aPeterziel, Heike$$b24$$udkfz
000178967 7001_ $$0P:(DE-He78)143af26de9d57bf624771616318aaf7c$$aWitt, Olaf$$b25$$udkfz
000178967 7001_ $$0P:(DE-He78)908367a659dea9e28dac34592b3c46e5$$aOehme, Ina$$b26$$eLast author$$udkfz
000178967 773__ $$0PERI:(DE-600)2527080-1$$a10.3390/cancers14030849$$gVol. 14, no. 3, p. 849 -$$n3$$p849$$tCancers$$v14$$x2072-6694$$y2022
000178967 909CO $$ooai:inrepo02.dkfz.de:178967$$pVDB
000178967 9101_ $$0I:(DE-588b)2036810-0$$6P:(DE-He78)35c44ba0995477242f21a01ca69d6945$$aDeutsches Krebsforschungszentrum$$b0$$kDKFZ
000178967 9101_ $$0I:(DE-588b)2036810-0$$6P:(DE-He78)53112ef656923758316e7079710bc988$$aDeutsches Krebsforschungszentrum$$b1$$kDKFZ
000178967 9101_ $$0I:(DE-588b)2036810-0$$6P:(DE-He78)ea170691ed65b95f10820c4c61d6e2cd$$aDeutsches Krebsforschungszentrum$$b2$$kDKFZ
000178967 9101_ $$0I:(DE-588b)2036810-0$$6P:(DE-He78)4302629f88822e785a2ba95b985cc952$$aDeutsches Krebsforschungszentrum$$b3$$kDKFZ
000178967 9101_ $$0I:(DE-588b)2036810-0$$6P:(DE-He78)2bebef4e65bbcb341e31ca0999b69436$$aDeutsches Krebsforschungszentrum$$b4$$kDKFZ
000178967 9101_ $$0I:(DE-588b)2036810-0$$6P:(DE-He78)b9b209c7129285852f61f807f332725a$$aDeutsches Krebsforschungszentrum$$b5$$kDKFZ
000178967 9101_ $$0I:(DE-588b)2036810-0$$6P:(DE-He78)3c021852cf94cb29825e65baa82ed54b$$aDeutsches Krebsforschungszentrum$$b6$$kDKFZ
000178967 9101_ $$0I:(DE-588b)2036810-0$$6P:(DE-He78)5ebf27c73a3f0b66abe1c471997cebb8$$aDeutsches Krebsforschungszentrum$$b7$$kDKFZ
000178967 9101_ $$0I:(DE-588b)2036810-0$$6P:(DE-He78)21d657d65d4ad0fd8b0bbfe10fa65172$$aDeutsches Krebsforschungszentrum$$b8$$kDKFZ
000178967 9101_ $$0I:(DE-588b)2036810-0$$6P:(DE-He78)311fe8118b7563b455ba84e94fccb3a8$$aDeutsches Krebsforschungszentrum$$b9$$kDKFZ
000178967 9101_ $$0I:(DE-588b)2036810-0$$6P:(DE-He78)32e22d1c8416af3f2a612da9121f2c05$$aDeutsches Krebsforschungszentrum$$b10$$kDKFZ
000178967 9101_ $$0I:(DE-588b)2036810-0$$6P:(DE-He78)f08873535c440e3ce033c84d5786f70f$$aDeutsches Krebsforschungszentrum$$b11$$kDKFZ
000178967 9101_ $$0I:(DE-588b)2036810-0$$6P:(DE-He78)2214defe87d04a849413e5eed47c415e$$aDeutsches Krebsforschungszentrum$$b12$$kDKFZ
000178967 9101_ $$0I:(DE-588b)2036810-0$$6P:(DE-He78)d5149ffd74f42a2fa87a086d66645aaa$$aDeutsches Krebsforschungszentrum$$b15$$kDKFZ
000178967 9101_ $$0I:(DE-588b)2036810-0$$6P:(DE-He78)8de7015af76bd61fb0bc13bb3facb1df$$aDeutsches Krebsforschungszentrum$$b16$$kDKFZ
000178967 9101_ $$0I:(DE-588b)2036810-0$$6P:(DE-He78)2f592d9d8339bee07cca3956b7472b61$$aDeutsches Krebsforschungszentrum$$b17$$kDKFZ
000178967 9101_ $$0I:(DE-588b)2036810-0$$6P:(DE-He78)261d2b84aded878003e6e35d18113831$$aDeutsches Krebsforschungszentrum$$b18$$kDKFZ
000178967 9101_ $$0I:(DE-588b)2036810-0$$6P:(DE-He78)551bb92841f634070997aa168d818492$$aDeutsches Krebsforschungszentrum$$b19$$kDKFZ
000178967 9101_ $$0I:(DE-588b)2036810-0$$6P:(DE-He78)990de6ec6c2f0a40b17ed75162ca19e8$$aDeutsches Krebsforschungszentrum$$b20$$kDKFZ
000178967 9101_ $$0I:(DE-588b)2036810-0$$6P:(DE-He78)91f32735ee876c579d63c05a7f4778dd$$aDeutsches Krebsforschungszentrum$$b21$$kDKFZ
000178967 9101_ $$0I:(DE-588b)2036810-0$$6P:(DE-He78)55dfd5b374ab9bccdb49e06e91345571$$aDeutsches Krebsforschungszentrum$$b22$$kDKFZ
000178967 9101_ $$0I:(DE-588b)2036810-0$$6P:(DE-He78)0be2f86573954f87e97f8a4dbb05cb0f$$aDeutsches Krebsforschungszentrum$$b23$$kDKFZ
000178967 9101_ $$0I:(DE-588b)2036810-0$$6P:(DE-He78)2727b5cb63b52d0137d4f4e8f110ee7e$$aDeutsches Krebsforschungszentrum$$b24$$kDKFZ
000178967 9101_ $$0I:(DE-588b)2036810-0$$6P:(DE-He78)143af26de9d57bf624771616318aaf7c$$aDeutsches Krebsforschungszentrum$$b25$$kDKFZ
000178967 9101_ $$0I:(DE-588b)2036810-0$$6P:(DE-He78)908367a659dea9e28dac34592b3c46e5$$aDeutsches Krebsforschungszentrum$$b26$$kDKFZ
000178967 9131_ $$0G:(DE-HGF)POF4-312$$1G:(DE-HGF)POF4-310$$2G:(DE-HGF)POF4-300$$3G:(DE-HGF)POF4$$4G:(DE-HGF)POF$$aDE-HGF$$bGesundheit$$lKrebsforschung$$vFunktionelle und strukturelle Genomforschung$$x0
000178967 9141_ $$y2022
000178967 915__ $$0LIC:(DE-HGF)CCBYNV$$2V:(DE-HGF)$$aCreative Commons Attribution CC BY (No Version)$$bDOAJ$$d2021-05-04
000178967 915__ $$0StatID:(DE-HGF)0160$$2StatID$$aDBCoverage$$bEssential Science Indicators$$d2021-05-04
000178967 915__ $$0StatID:(DE-HGF)1190$$2StatID$$aDBCoverage$$bBiological Abstracts$$d2021-05-04
000178967 915__ $$0StatID:(DE-HGF)0113$$2StatID$$aWoS$$bScience Citation Index Expanded$$d2021-05-04
000178967 915__ $$0StatID:(DE-HGF)0561$$2StatID$$aArticle Processing Charges$$d2021-05-04
000178967 915__ $$0StatID:(DE-HGF)0700$$2StatID$$aFees$$d2021-05-04
000178967 915__ $$0StatID:(DE-HGF)0100$$2StatID$$aJCR$$bCANCERS : 2021$$d2022-11-30
000178967 915__ $$0StatID:(DE-HGF)0200$$2StatID$$aDBCoverage$$bSCOPUS$$d2022-11-30
000178967 915__ $$0StatID:(DE-HGF)0300$$2StatID$$aDBCoverage$$bMedline$$d2022-11-30
000178967 915__ $$0StatID:(DE-HGF)0501$$2StatID$$aDBCoverage$$bDOAJ Seal$$d2022-01-24T07:56:58Z
000178967 915__ $$0StatID:(DE-HGF)0500$$2StatID$$aDBCoverage$$bDOAJ$$d2022-01-24T07:56:58Z
000178967 915__ $$0StatID:(DE-HGF)0030$$2StatID$$aPeer Review$$bDOAJ : Blind peer review$$d2022-01-24T07:56:58Z
000178967 915__ $$0StatID:(DE-HGF)0600$$2StatID$$aDBCoverage$$bEbsco Academic Search$$d2022-11-30
000178967 915__ $$0StatID:(DE-HGF)0030$$2StatID$$aPeer Review$$bASC$$d2022-11-30
000178967 915__ $$0StatID:(DE-HGF)0199$$2StatID$$aDBCoverage$$bClarivate Analytics Master Journal List$$d2022-11-30
000178967 915__ $$0StatID:(DE-HGF)0150$$2StatID$$aDBCoverage$$bWeb of Science Core Collection$$d2022-11-30
000178967 915__ $$0StatID:(DE-HGF)1050$$2StatID$$aDBCoverage$$bBIOSIS Previews$$d2022-11-30
000178967 915__ $$0StatID:(DE-HGF)9905$$2StatID$$aIF >= 5$$bCANCERS : 2021$$d2022-11-30
000178967 9202_ $$0I:(DE-He78)B310-20160331$$kB310$$lKKE Pädiatrische Onkologie$$x0
000178967 9201_ $$0I:(DE-He78)B310-20160331$$kB310$$lKKE Pädiatrische Onkologie$$x0
000178967 9201_ $$0I:(DE-He78)HD01-20160331$$kHD01$$lDKTK HD zentral$$x1
000178967 9201_ $$0I:(DE-He78)B240-20160331$$kB240$$lB240 Bioinformatik und Omics Data Analytics$$x2
000178967 9201_ $$0I:(DE-He78)B062-20160331$$kB062$$lB062 Pädiatrische Neuroonkologie$$x3
000178967 9201_ $$0I:(DE-He78)B380-20160331$$kB380$$lNWG Weichteil-Sarkome$$x4
000178967 9201_ $$0I:(DE-He78)B087-20160331$$kB087$$lB087 Neuroblastom Genomik$$x5
000178967 9201_ $$0I:(DE-He78)W210-20160331$$kW210$$lLichtmikroskopie$$x6
000178967 9201_ $$0I:(DE-He78)B300-20160331$$kB300$$lKKE Neuropathologie$$x7
000178967 9201_ $$0I:(DE-He78)B360-20160331$$kB360$$lPediatric Glioma$$x8
000178967 9200_ $$0I:(DE-He78)B310-20160331$$kB310$$lKKE Pädiatrische Onkologie$$x0
000178967 980__ $$ajournal
000178967 980__ $$aVDB
000178967 980__ $$aI:(DE-He78)B310-20160331
000178967 980__ $$aI:(DE-He78)HD01-20160331
000178967 980__ $$aI:(DE-He78)B240-20160331
000178967 980__ $$aI:(DE-He78)B062-20160331
000178967 980__ $$aI:(DE-He78)B380-20160331
000178967 980__ $$aI:(DE-He78)B087-20160331
000178967 980__ $$aI:(DE-He78)W210-20160331
000178967 980__ $$aI:(DE-He78)B300-20160331
000178967 980__ $$aI:(DE-He78)B360-20160331
000178967 980__ $$aUNRESTRICTED