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@ARTICLE{Kaltenbacher:179131,
      author       = {T. Kaltenbacher and J. Löprich and R. Maresch and J. Weber
                      and S. Müller and R. Oellinger and N. Groß and J. Griger
                      and N. de Andrade Krätzig and P. Avramopoulos and D.
                      Ramanujam and S. Brummer and S. A. Widholz and S. Bärthel
                      and C. Falcomatà and A. Pfaus and A. Alnatsha and J.
                      Mayerle$^*$ and M. Schmidt-Supprian$^*$ and M. Reichert and
                      G. Schneider and U. Ehmer and C. J. Braun$^*$ and D. K. M.
                      Saur$^*$ and S. Engelhardt and R. Rad$^*$},
      title        = {{CRISPR} somatic genome engineering and cancer modeling in
                      the mouse pancreas and liver.},
      journal      = {Nature protocols},
      volume       = {17},
      number       = {4},
      issn         = {1750-2799},
      address      = {Basingstoke},
      publisher    = {Nature Publishing Group},
      reportid     = {DKFZ-2022-00488},
      pages        = {1142-1188},
      year         = {2022},
      note         = {2022 Apr;17(4):1142-1188},
      abstract     = {Genetically engineered mouse models (GEMMs) transformed the
                      study of organismal disease phenotypes but are limited by
                      their lengthy generation in embryonic stem cells. Here, we
                      describe methods for rapid and scalable genome engineering
                      in somatic cells of the liver and pancreas through delivery
                      of CRISPR components into living mice. We introduce the
                      spectrum of genetic tools, delineate viral and nonviral
                      CRISPR delivery strategies and describe a series of
                      applications, ranging from gene editing and cancer modeling
                      to chromosome engineering or CRISPR multiplexing and its
                      spatio-temporal control. Beyond experimental design and
                      execution, the protocol describes quantification of genetic
                      and functional editing outcomes, including sequencing
                      approaches, data analysis and interpretation. Compared to
                      traditional knockout mice, somatic GEMMs face an increased
                      risk for mouse-to-mouse variability because of the higher
                      experimental demands of the procedures. The robust protocols
                      described here will help unleash the full potential of
                      somatic genome manipulation. Depending on the delivery
                      method and envisaged application, the protocol takes 3-5
                      weeks.},
      subtyp        = {Review Article},
      cin          = {MU01 / B062},
      ddc          = {610},
      cid          = {I:(DE-He78)MU01-20160331 / I:(DE-He78)B062-20160331},
      pnm          = {312 - Funktionelle und strukturelle Genomforschung
                      (POF4-312)},
      pid          = {G:(DE-HGF)POF4-312},
      typ          = {PUB:(DE-HGF)16},
      pubmed       = {pmid:35288718},
      doi          = {10.1038/s41596-021-00677-0},
      url          = {https://inrepo02.dkfz.de/record/179131},
}