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@ARTICLE{Sowah:179134,
author = {S. Sowah$^*$ and A. Milanese and R. Schübel$^*$ and J.
Wirbel and E. Kartal and T. S. Johnson$^*$ and F. Hirche and
M. Grafetstätter$^*$ and T. Nonnenmacher and R. Kirsten and
M. López-Nogueroles and A. Lahoz and K. V. Schwarz and J.
G. Okun and C. M. Ulrich and J. Nattenmüller and A. von
Eckardstein and D. Müller and G. I. Stangl and R. Kaaks$^*$
and T. Kühn$^*$ and G. Zeller},
title = {{C}alorie restriction improves metabolic state
independently of gut microbiome composition: a randomized
dietary intervention trial.},
journal = {Genome medicine},
volume = {14},
number = {1},
issn = {1756-994X},
address = {London},
publisher = {BioMed Central},
reportid = {DKFZ-2022-00491},
pages = {30},
year = {2022},
note = {#EA:C020#LA:C020#},
abstract = {The gut microbiota has been suggested to play a significant
role in the development of overweight and obesity. However,
the effects of calorie restriction on gut microbiota of
overweight and obese adults, especially over longer
durations, are largely unexplored.Here, we longitudinally
analyzed the effects of intermittent calorie restriction
(ICR) operationalized as the 5:2 diet versus continuous
calorie restriction (CCR) on fecal microbiota of 147
overweight or obese adults in a 50-week parallel-arm
randomized controlled trial, the HELENA Trial. The primary
outcome of the trial was the differential effects of ICR
versus CCR on gene expression in subcutaneous adipose
tissue. Changes in the gut microbiome, which are the focus
of this publication, were defined as exploratory endpoint of
the trial. The trial comprised a 12-week intervention
period, a 12-week maintenance period, and a final follow-up
period of 26 weeks.Both diets resulted in $~5\%$ weight
loss. However, except for Lactobacillales being enriched
after ICR, post-intervention microbiome composition did not
significantly differ between groups. Overall weight loss was
associated with significant metabolic improvements, but not
with changes in the gut microbiome. Nonetheless, the
abundance of the Dorea genus at baseline was moderately
predictive of subsequent weight loss (AUROC of 0.74 for
distinguishing the highest versus lowest weight loss
quartiles). Despite the lack of consistent intervention
effects on microbiome composition, significant study
group-independent co-variation between gut bacterial
families and metabolic biomarkers, anthropometric measures,
and dietary composition was detectable. Our analysis in
particular revealed associations between insulin sensitivity
(HOMA-IR) and Akkermansiaceae, Christensenellaceae, and
Tanerellaceae. It also suggests the possibility of a
beneficial modulation of the latter two intestinal taxa by a
diet high in vegetables and fiber, and low in processed
meat.Overall, our results suggest that the gut microbiome
remains stable and highly individual-specific under dietary
calorie restriction.The trial, including the present
microbiome component, was prospectively registered at
ClinicalTrials.gov NCT02449148 on May 20, 2015.},
keywords = {Gut microbiome (Other) / Intermittent calorie restriction
(Other) / Obesity (Other) / Overweight (Other) / Weight loss
(Other)},
cin = {C020},
ddc = {610},
cid = {I:(DE-He78)C020-20160331},
pnm = {313 - Krebsrisikofaktoren und Prävention (POF4-313)},
pid = {G:(DE-HGF)POF4-313},
typ = {PUB:(DE-HGF)16},
pubmed = {pmid:35287713},
doi = {10.1186/s13073-022-01030-0},
url = {https://inrepo02.dkfz.de/record/179134},
}