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@ARTICLE{Bhardwaj:179284,
      author       = {M. Bhardwaj$^*$ and P. Schrotz-King$^*$ and H. Brenner$^*$},
      title        = {{C}orrelation of {R}epeat {M}easurements of 27 {C}andidate
                      {P}rotein {M}arkers for {C}olorectal {C}ancer {S}creening
                      {T}aken {T}hree {Y}ears and {M}ultiple {F}reeze-{T}haw
                      {C}ycles {A}part.},
      journal      = {Life},
      volume       = {12},
      number       = {3},
      issn         = {2075-1729},
      address      = {Basel},
      publisher    = {MDPI},
      reportid     = {DKFZ-2022-00566},
      pages        = {359},
      year         = {2022},
      note         = {#EA:C070#LA:C070#LA:C120#},
      abstract     = {In recent years the blood proteome has been increasingly
                      researched for biomarkers for early detection of colorectal
                      cancer (CRC). Blood samples from screening studies are often
                      subject to preanalytical variability and repeated
                      freeze-thaw cycles. We aimed to assess the correlation of
                      repeat measurements of 27 candidate protein markers for CRC
                      screening taken three years and multiple freeze-thaw cycles
                      apart. The concentrations of 27 protein markers were
                      measured in plasma samples of 39 newly detected CRC cases
                      from a cohort of 9245 participants of screening
                      colonoscopies. The proteins were measured using proximity
                      extension assays (PEA) carried out on the same set of
                      samples twice, three years apart, with an average of three
                      freeze-thaw cycles in between the two measurements.
                      Pearson's product moment correlation coefficients were
                      calculated. Correlation coefficients ranged from +0.43 to
                      +0.97, with a median of 0.67 and an interquartile range of
                      +0.58 to +0.84, with all p-values of correlation being <0.01
                      (<0.0005 for 22 proteins, <0.001 for 4 proteins). Repeat
                      measurements of the 27 protein biomarkers for CRC screening
                      performed three years later, and on average three
                      freeze-thaw cycles apart, showed moderate to high levels of
                      correlation. Apart from the effects of freeze-thaw cycles,
                      slightly different preprocessing performed on the data may
                      have contributed to recorded differences between
                      measurements.},
      keywords     = {colorectal cancer (Other) / freeze–thaw cycles (Other) /
                      plasma proteins (Other) / proximity extension assays (Other)
                      / sample handling (Other)},
      cin          = {C070 / C120 / HD01},
      ddc          = {570},
      cid          = {I:(DE-He78)C070-20160331 / I:(DE-He78)C120-20160331 /
                      I:(DE-He78)HD01-20160331},
      pnm          = {313 - Krebsrisikofaktoren und Prävention (POF4-313)},
      pid          = {G:(DE-HGF)POF4-313},
      typ          = {PUB:(DE-HGF)16},
      pubmed       = {pmid:35330110},
      pmc          = {pmc:PMC8949042},
      doi          = {10.3390/life12030359},
      url          = {https://inrepo02.dkfz.de/record/179284},
}