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@ARTICLE{Schttker:179339,
      author       = {B. Schöttker$^*$ and E. L. Larsen and A. Weimann and T.
                      Henriksen and H. Brenner$^*$ and H. E. Poulsen},
      title        = {{A}ssociations of urinary metabolites of oxidized {DNA} and
                      {RNA} with the incidence of diabetes mellitus using
                      {UPLC}-{MS}/{MS} and {ELISA} methods.},
      journal      = {Free radical biology and medicine},
      volume       = {183},
      issn         = {0891-5849},
      address      = {New York, NY [u.a.]},
      publisher    = {Elsevier},
      reportid     = {DKFZ-2022-00605},
      pages        = {51 - 59},
      year         = {2022},
      note         = {#EA:C070#},
      abstract     = {To evaluate the association of urinary oxidized
                      guanine/guanosine (OxGuo) levels with incident type 2
                      diabetes (T2D) among older adults.A nested case-control
                      design was applied with 440 cases of incident T2D and 440
                      controls, randomly sampled from all 65-75 year-old study
                      participants of the ESTHER study, which is a
                      population-based German cohort study with 14 years of
                      follow-up. Analyses of 8-hydroxy-2'-deoxyguanosine
                      (8-oxo-dGuo; DNA oxidation product) and 8-hydroxyguanosine
                      (8-oxo-Guo; RNA oxidation product) were measured by
                      ultra-performance liquid chromatography tandem-mass
                      spectrometry (UPLC-MS/MS). The sum of the two OxGuo molecule
                      concentrations was calculated and called OxGuo-UPLC-MS/MS.
                      The corresponding OxGuo-ELISA levels were measured by
                      Cayman's DNA/RNA oxidative damage ELISA, which detects a mix
                      of 8-oxo-dGuo, 8-oxo-Guo and one other OxGuo molecule.
                      Logistic regression was applied and models were adjusted for
                      age, sex, BMI, HbA1c, and C-reactive protein
                      levels.8-oxo-dGuo and 8-oxo-Guo were highly correlated with
                      each other (r = 0.642) and weakly correlated with
                      OxGuo-ELISA (r = 0.22 and r = 0.14, respectively).
                      OxGuo-ELISA levels were statistically significant associated
                      with T2D incidence (odds ratio (OR) and $95\%$ confidence
                      interval $[95\%CI]$ for comparison of top and bottom
                      quartile: 1.77 [1.14; 2.76]). In contrast, the ORs did not
                      increase stepwise from quartile 2 to 4 for neither
                      8-oxo-Guo, 8-oxo-dGuo levels nor OxGuo-UPLC-MS/MS and
                      comparisons of top and bottom quartile were not
                      statistically significant. In a post-hoc analysis comparing
                      bottom quartile 1 with a combined group of quartile 2-4, the
                      association of OxGuo-UPLC-MS/MS with T2D incidence reached
                      statistical significance (OR $[95\%CI]:$ 0.66 [0.46; 0.96])
                      and was very similar with the one obtained for OxGuo-ELISA
                      (OR $[95\%CI]:$ 0.66 [0.45; 0.95]).Although only the
                      measurements of the DNA/RNA oxidative damage ELISA kit of
                      Cayman were statistically significantly associated with T2D
                      incidence in the main analysis, confidence intervals
                      overlapped and the post-hoc analysis showed that results for
                      OxGuo-UPLC-MS/MS were quite comparable.},
      keywords     = {8-Hydroxy-2′-deoxyguanosine (Other) / Cohort study
                      (Other) / Diabetes mellitus (Other) / Free radicals (Other)
                      / Oxidative stress (Other) / Type 2 (Other)},
      cin          = {C070},
      ddc          = {610},
      cid          = {I:(DE-He78)C070-20160331},
      pnm          = {313 - Krebsrisikofaktoren und Prävention (POF4-313)},
      pid          = {G:(DE-HGF)POF4-313},
      typ          = {PUB:(DE-HGF)16},
      pubmed       = {pmid:35307553},
      doi          = {10.1016/j.freeradbiomed.2022.03.007},
      url          = {https://inrepo02.dkfz.de/record/179339},
}