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@ARTICLE{Khalid:179901,
author = {U. Khalid$^*$ and M. Simovic$^*$ and L. A. Hammann$^*$ and
M. Iskar$^*$ and J. K. L. Wong$^*$ and R. Kumar$^*$ and M.
Jugold$^*$ and M. Sill$^*$ and M. Bolkestein$^*$ and T.
Kolb$^*$ and M. Hergt$^*$ and F. Devens$^*$ and J. Ecker$^*$
and M. Kool$^*$ and T. Milde$^*$ and F. Westermann$^*$ and
A. Benner$^*$ and J. Lewis and S. Dietrich and S. M.
Pfister$^*$ and P. Lichter$^*$ and M. Zapatka$^*$ and A.
Ernst$^*$},
title = {{A} synergistic interaction between {HDAC}- and {PARP}
inhibitors in childhood tumors with chromothripsis.},
journal = {International journal of cancer},
volume = {151},
number = {4},
issn = {0020-7136},
address = {Bognor Regis},
publisher = {Wiley-Liss},
reportid = {DKFZ-2022-00958},
pages = {590-606},
year = {2022},
note = {#EA:B420#LA:B420# / 2022 Aug 15;151(4):590-606},
abstract = {Chromothripsis is a form of genomic instability
characterized by the occurrence of tens to hundreds of
clustered DNA double-strand breaks in a one-off catastrophic
event. Rearrangements associated with chromothripsis are
detectable in numerous tumor entities and linked with poor
prognosis in some of these, such as Sonic Hedgehog
medulloblastoma, neuroblastoma and osteosarcoma. Hence,
there is a need for therapeutic strategies eliminating tumor
cells with chromothripsis. Defects in DNA double-strand
break repair, and in particular homologous recombination
repair, have been linked with chromothripsis. Targeting DNA
repair deficiencies by synthetic lethality approaches, we
performed a synergy screen using drug libraries (n = 375
compounds, 15 models) combined with either a PARP inhibitor
or cisplatin. This revealed a synergistic interaction
between the HDAC inhibitor romidepsin and PARP inhibition.
Functional assays, transcriptome analyses and in vivo
validation in patient-derived xenograft mouse models
confirmed the efficacy of the combinatorial treatment.},
keywords = {HDAC inhibitor (Other) / PARP inhibitor (Other) /
chromothripsis (Other) / synergy (Other) / synthetic
lethality (Other)},
cin = {B420 / B060 / W240 / B062 / HD01 / B310 / B087 / C060},
ddc = {610},
cid = {I:(DE-He78)B420-20160331 / I:(DE-He78)B060-20160331 /
I:(DE-He78)W240-20160331 / I:(DE-He78)B062-20160331 /
I:(DE-He78)HD01-20160331 / I:(DE-He78)B310-20160331 /
I:(DE-He78)B087-20160331 / I:(DE-He78)C060-20160331},
pnm = {312 - Funktionelle und strukturelle Genomforschung
(POF4-312)},
pid = {G:(DE-HGF)POF4-312},
typ = {PUB:(DE-HGF)16},
pubmed = {pmid:35411591},
doi = {10.1002/ijc.34027},
url = {https://inrepo02.dkfz.de/record/179901},
}