TY  - JOUR
AU  - Mikhnevich, Tatyana A.
AU  - Grigorenko, Vitaly G.
AU  - Rubtsova, Maya Yu.
AU  - Rukhovich, Gleb
AU  - Letarova, Maria A.
AU  - Kravtsova, Darya S.
AU  - Vladimirov, Sergey A.
AU  - Orlov, Alexey A.
AU  - Nikolaev, Evgeny N.
AU  - Zherebker, Alexander
AU  - Perminova, Irina V.
TI  - Inhibition of Class A β-Lactamase (TEM-1) by Narrow Fractions of Humic Substances
JO  - ACS omega
VL  - 6
IS  - 37
SN  - 2470-1343
CY  - Washington, DC
PB  - ACS Publications
M1  - DKFZ-2022-01702
SP  - 23873 - 23883
PY  - 2021
AB  - Antimicrobial resistance is a global threat. The use of biologically active natural products alone or in combination with the clinically proven antimicrobial agents might be a useful strategy to fight the resistance. The scientific hypotheses of this study were twofold: (1) the natural humic substances rich in dicarboxyl, phenolic, heteroaryl, and other fragments might possess inhibitory activity against β-lactamases, and (2) this inhibitory activity might be linked to the molecular composition of the humic ensemble. To test these hypotheses, we used humic substances (HS) from different sources (coal, peat, and soil) and of different fractional compositions (humic acids, hymatomelanic acids, and narrow fractions from solid-phase extraction) for inhibiting serine β-lactamase TEM-1. Fourier transform ion cyclotron resonance mass spectrometry (FTICR MS) was used to characterize the molecular composition of all humic materials used in this study. The kinetic assay with chromogenic substrate CENTA was used for assessment of inhibitory activity. The inhibition data have shown that among all humic materials tested, a distinct activity was observed within apolar fractions of hymatomelanic acid isolated from lignite. The decrease in the hydrolysis rate in the presence of most active fractions was 42
LB  - PUB:(DE-HGF)16
DO  - DOI:10.1021/acsomega.1c02841
UR  - https://inrepo02.dkfz.de/record/180977
ER  -