%0 Journal Article
%A Möhrmann, Lino
%A Huang, Helen J
%A Hong, David S
%A Tsimberidou, Apostolia M
%A Fu, Siqing
%A Piha-Paul, Sarina A
%A Subbiah, Vivek
%A Karp, Daniel D
%A Naing, Aung
%A Krug, Anne
%A Enderle, Daniel
%A Priewasser, Tina
%A Noerholm, Mikkel
%A Eitan, Erez
%A Coticchia, Christine
%A Stoll, Georg
%A Jordan, Lisa-Marie
%A Eng, Cathy
%A Kopetz, E Scott
%A Skog, Johan
%A Meric-Bernstam, Funda
%A Janku, Filip
%T Liquid Biopsies Using Plasma Exosomal Nucleic Acids and Plasma Cell-Free DNA Compared with Clinical Outcomes of Patients with Advanced Cancers.
%J Clinical cancer research
%V 24
%N 1
%@ 1078-0432
%C Philadelphia, Pa. [u.a.]
%I AACR
%M DKFZ-2022-01956
%P 181 - 188
%D 2018
%Z POF Topic: 317
%X Purpose: Blood-based liquid biopsies offer easy access to genomic material for molecular diagnostics in cancer. Commonly used cell-free DNA (cfDNA) originates from dying cells. Exosomal nucleic acids (exoNAs) originate from living cells, which can better reflect underlying cancer biology.Experimental Design: Next-generation sequencing (NGS) was used to test exoNA, and droplet digital PCR (ddPCR) and BEAMing PCR were used to test cfDNA for BRAFV600, KRASG12/G13, and EGFRexon19del/L858R mutations in 43 patients with progressing advanced cancers. Results were compared with clinical testing of archival tumor tissue and clinical outcomes.Results: Forty-one patients had BRAF, KRAS, or EGFR mutations in tumor tissue. These mutations were detected by NGS in 95
%K Adult
%K Aged
%K Biomarkers, Tumor
%K Cell-Free Nucleic Acids
%K ErbB Receptors: blood
%K ErbB Receptors: genetics
%K Exosomes
%K Female
%K Genetic Testing
%K Humans
%K Liquid Biopsy: methods
%K Male
%K Middle Aged
%K Mutation
%K Neoplasm Grading
%K Neoplasm Staging
%K Neoplasms: blood
%K Neoplasms: diagnosis
%K Neoplasms: mortality
%K Patient Outcome Assessment
%K Proto-Oncogene Proteins B-raf: blood
%K Proto-Oncogene Proteins B-raf: genetics
%K Proto-Oncogene Proteins p21(ras): blood
%K Proto-Oncogene Proteins p21(ras): genetics
%K Survival Analysis
%K Biomarkers, Tumor (NLM Chemicals)
%K Cell-Free Nucleic Acids (NLM Chemicals)
%K KRAS protein, human (NLM Chemicals)
%K EGFR protein, human (NLM Chemicals)
%K ErbB Receptors (NLM Chemicals)
%K BRAF protein, human (NLM Chemicals)
%K Proto-Oncogene Proteins B-raf (NLM Chemicals)
%K Proto-Oncogene Proteins p21(ras) (NLM Chemicals)
%F PUB:(DE-HGF)16
%9 Journal Article
%$ pmid:29051321
%2 pmc:PMC5754253
%R 10.1158/1078-0432.CCR-17-2007
%U https://inrepo02.dkfz.de/record/181329