TY  - JOUR
AU  - Möhrmann, Lino
AU  - Huang, Helen J
AU  - Hong, David S
AU  - Tsimberidou, Apostolia M
AU  - Fu, Siqing
AU  - Piha-Paul, Sarina A
AU  - Subbiah, Vivek
AU  - Karp, Daniel D
AU  - Naing, Aung
AU  - Krug, Anne
AU  - Enderle, Daniel
AU  - Priewasser, Tina
AU  - Noerholm, Mikkel
AU  - Eitan, Erez
AU  - Coticchia, Christine
AU  - Stoll, Georg
AU  - Jordan, Lisa-Marie
AU  - Eng, Cathy
AU  - Kopetz, E Scott
AU  - Skog, Johan
AU  - Meric-Bernstam, Funda
AU  - Janku, Filip
TI  - Liquid Biopsies Using Plasma Exosomal Nucleic Acids and Plasma Cell-Free DNA Compared with Clinical Outcomes of Patients with Advanced Cancers.
JO  - Clinical cancer research
VL  - 24
IS  - 1
SN  - 1078-0432
CY  - Philadelphia, Pa. [u.a.]
PB  - AACR
M1  - DKFZ-2022-01956
SP  - 181 - 188
PY  - 2018
N1  - POF Topic: 317
AB  - Purpose: Blood-based liquid biopsies offer easy access to genomic material for molecular diagnostics in cancer. Commonly used cell-free DNA (cfDNA) originates from dying cells. Exosomal nucleic acids (exoNAs) originate from living cells, which can better reflect underlying cancer biology.Experimental Design: Next-generation sequencing (NGS) was used to test exoNA, and droplet digital PCR (ddPCR) and BEAMing PCR were used to test cfDNA for BRAFV600, KRASG12/G13, and EGFRexon19del/L858R mutations in 43 patients with progressing advanced cancers. Results were compared with clinical testing of archival tumor tissue and clinical outcomes.Results: Forty-one patients had BRAF, KRAS, or EGFR mutations in tumor tissue. These mutations were detected by NGS in 95
KW  - Adult
KW  - Aged
KW  - Biomarkers, Tumor
KW  - Cell-Free Nucleic Acids
KW  - ErbB Receptors: blood
KW  - ErbB Receptors: genetics
KW  - Exosomes
KW  - Female
KW  - Genetic Testing
KW  - Humans
KW  - Liquid Biopsy: methods
KW  - Male
KW  - Middle Aged
KW  - Mutation
KW  - Neoplasm Grading
KW  - Neoplasm Staging
KW  - Neoplasms: blood
KW  - Neoplasms: diagnosis
KW  - Neoplasms: mortality
KW  - Patient Outcome Assessment
KW  - Proto-Oncogene Proteins B-raf: blood
KW  - Proto-Oncogene Proteins B-raf: genetics
KW  - Proto-Oncogene Proteins p21(ras): blood
KW  - Proto-Oncogene Proteins p21(ras): genetics
KW  - Survival Analysis
KW  - Biomarkers, Tumor (NLM Chemicals)
KW  - Cell-Free Nucleic Acids (NLM Chemicals)
KW  - KRAS protein, human (NLM Chemicals)
KW  - EGFR protein, human (NLM Chemicals)
KW  - ErbB Receptors (NLM Chemicals)
KW  - BRAF protein, human (NLM Chemicals)
KW  - Proto-Oncogene Proteins B-raf (NLM Chemicals)
KW  - Proto-Oncogene Proteins p21(ras) (NLM Chemicals)
LB  - PUB:(DE-HGF)16
C6  - pmid:29051321
C2  - pmc:PMC5754253
DO  - DOI:10.1158/1078-0432.CCR-17-2007
UR  - https://inrepo02.dkfz.de/record/181329
ER  -