% IMPORTANT: The following is UTF-8 encoded.  This means that in the presence
% of non-ASCII characters, it will not work with BibTeX 0.99 or older.
% Instead, you should use an up-to-date BibTeX implementation like “bibtex8” or
% “biber”.

@ARTICLE{Grimm:181836,
      author       = {E. Grimm$^*$ and F. van der Hoeven$^*$ and D. Sardella and
                      K. I. Willig and U. Engel and N. Veits and R. Engel$^*$ and
                      E. A. Cavalcanti-Adam and F. Bestvater$^*$ and L. Bordoni
                      and R. Jennemann$^*$ and K. Schönig and I. M. Schiessl and
                      R. Sandhoff$^*$},
      title        = {{A} {C}lathrin light chain {A} reporter mouse for in vivo
                      imaging of endocytosis.},
      journal      = {PLOS ONE},
      volume       = {17},
      number       = {9},
      issn         = {1932-6203},
      address      = {San Francisco, California, US},
      publisher    = {PLOS},
      reportid     = {DKFZ-2022-02251},
      pages        = {e0273660 -},
      year         = {2022},
      note         = {#EA:A411#LA:A411#},
      abstract     = {Clathrin-mediated endocytosis (CME) is one of the best
                      studied cellular uptake pathways and its contributions to
                      nutrient uptake, receptor signaling, and maintenance of the
                      lipid membrane homeostasis have been already elucidated.
                      Today, we still have a lack of understanding how the
                      different components of this pathway cooperate dynamically
                      in vivo. Therefore, we generated a reporter mouse model for
                      CME by fusing eGFP endogenously in frame to clathrin light
                      chain a (Clta) to track endocytosis in living mice. The
                      fusion protein is expressed in all tissues, but in a cell
                      specific manner, and can be visualized using fluorescence
                      microscopy. Recruitment to nanobeads recorded by TIRF
                      microscopy validated the functionality of the Clta-eGFP
                      reporter. With this reporter model we were able to track the
                      dynamics of Alexa594-BSA uptake in kidneys of anesthetized
                      mice using intravital 2-photon microscopy. This reporter
                      mouse model is not only a suitable and powerful tool to
                      track CME in vivo in genetic or disease mouse models it can
                      also help to shed light into the differential roles of the
                      two clathrin light chain isoforms in health and disease.},
      cin          = {A411 / W450 / W210},
      ddc          = {610},
      cid          = {I:(DE-He78)A411-20160331 / I:(DE-He78)W450-20160331 /
                      I:(DE-He78)W210-20160331},
      pnm          = {311 - Zellbiologie und Tumorbiologie (POF4-311)},
      pid          = {G:(DE-HGF)POF4-311},
      typ          = {PUB:(DE-HGF)16},
      pubmed       = {pmid:36149863},
      doi          = {10.1371/journal.pone.0273660},
      url          = {https://inrepo02.dkfz.de/record/181836},
}