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000181839 041__ $$aEnglish
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000181839 1001_ $$00000-0002-2795-597X$$aMaterniak-Kornas, Magdalena$$b0
000181839 245__ $$aOccurrence of Equine Foamy Virus Infection in Horses from Poland.
000181839 260__ $$aBasel$$bMDPI$$c2022
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000181839 520__ $$aEquine foamy virus (EFVeca) is a foamy virus of non-primate origin and among the least-studied members of this retroviral subfamily. By sequence comparison, EFVeca shows the highest similarity to bovine foamy virus. In contrast to simian, bovine or feline foamy viruses, knowledge about the epidemiology of EFVeca is still limited. Since preliminary studies suggested EFVeca infections among horses in Poland, we aimed to expand the diagnostics of EFVeca infections by developing specific diagnostic tools and apply them to investigate its prevalence. An ELISA test based on recombinant EFVeca Gag protein was developed for serological investigation, while semi-nested PCR for the detection of EFVeca DNA was established. 248 DNA and serum samples from purebred horses, livestock and saddle horses, Hucul horses and semi-feral Polish primitive horses were analyzed in this study. ELISA was standardized, and cut off value, sensitivity and specificity of the test were calculated using Receiver Operating Characteristic and Bayesian estimation. Based on the calculated cut off, 135 horses were seropositive to EFVeca Gag protein, while EFVeca proviral DNA was detected in 85 animals. The rate of infected individuals varied among the horse groups studied; this is the first report confirming the existence of EFVeca infections in horses from Poland using virus-specific tools.
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000181839 650_7 $$2Other$$aELISA
000181839 650_7 $$2Other$$aPCR
000181839 650_7 $$2Other$$adiagnostics
000181839 650_7 $$2Other$$aequine foamy virus
000181839 650_7 $$2Other$$ahorses
000181839 650_7 $$2Other$$aprevalence
000181839 7001_ $$aRożek, Wojciech$$b1
000181839 7001_ $$00000-0002-0615-4444$$aRola, Jerzy$$b2
000181839 7001_ $$aOsiński, Zbigniew$$b3
000181839 7001_ $$0P:(DE-He78)9c8a7dc019ff6a8d3da29f2069054a6f$$aLöchelt, Martin$$b4$$udkfz
000181839 7001_ $$aKuźmak, Jacek$$b5
000181839 773__ $$0PERI:(DE-600)2516098-9$$a10.3390/v14091973$$gVol. 14, no. 9, p. 1973 -$$n9$$p1973$$tViruses$$v14$$x1999-4915$$y2022
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