000181955 001__ 181955
000181955 005__ 20240229145702.0
000181955 0247_ $$2doi$$a10.3389/fcell.2022.954358
000181955 0247_ $$2pmid$$apmid:36187487
000181955 0247_ $$2pmc$$apmc:PMC9515781
000181955 0247_ $$2altmetric$$aaltmetric:135927765
000181955 037__ $$aDKFZ-2022-02325
000181955 041__ $$aEnglish
000181955 082__ $$a570
000181955 1001_ $$aSchwarz, Jessica Denise$$b0
000181955 245__ $$aThe glycolytic enzyme ALDOA and the exon junction complex protein RBM8A are regulators of ribosomal biogenesis.
000181955 260__ $$aLausanne$$bFrontiers Media$$c2022
000181955 3367_ $$2DRIVER$$aarticle
000181955 3367_ $$2DataCite$$aOutput Types/Journal article
000181955 3367_ $$0PUB:(DE-HGF)16$$2PUB:(DE-HGF)$$aJournal Article$$bjournal$$mjournal$$s1664969426_8112
000181955 3367_ $$2BibTeX$$aARTICLE
000181955 3367_ $$2ORCID$$aJOURNAL_ARTICLE
000181955 3367_ $$00$$2EndNote$$aJournal Article
000181955 520__ $$aCellular growth is a fundamental process of life and must be precisely controlled in multicellular organisms. Growth is crucially controlled by the number of functional ribosomes available in cells. The production of new ribosomes depends critically on the activity of RNA polymerase (RNAP) II in addition to the activity of RNAP I and III, which produce ribosomal RNAs. Indeed, the expression of both, ribosomal proteins and proteins required for ribosome assembly (ribosomal biogenesis factors), is considered rate-limiting for ribosome synthesis. Here, we used genetic screening to identify novel transcriptional regulators of cell growth genes by fusing promoters from a ribosomal protein gene (Rpl18) and from a ribosomal biogenesis factor (Fbl) with fluorescent protein genes (RFP, GFP) as reporters. Subsequently, both reporters were stably integrated into immortalized mouse fibroblasts, which were then transduced with a genome-wide sgRNA-CRISPR knockout library. Subsequently, cells with altered reporter activity were isolated by FACS and the causative sgRNAs were identified. Interestingly, we identified two novel regulators of growth genes. Firstly, the exon junction complex protein RBM8A controls transcript levels of the intronless reporters used here. By acute depletion of RBM8A protein using the auxin degron system combined with the genome-wide analysis of nascent transcription, we showed that RBM8A is an important global regulator of ribosomal protein transcripts. Secondly, we unexpectedly observed that the glycolytic enzyme aldolase A (ALDOA) regulates the expression of ribosomal biogenesis factors. Consistent with published observations that a fraction of this protein is located in the nucleus, this may be a mechanism linking transcription of growth genes to metabolic processes and possibly to metabolite availability.
000181955 536__ $$0G:(DE-HGF)POF4-311$$a311 - Zellbiologie und Tumorbiologie (POF4-311)$$cPOF4-311$$fPOF IV$$x0
000181955 588__ $$aDataset connected to CrossRef, PubMed, , Journals: inrepo02.dkfz.de
000181955 650_7 $$2Other$$aAldoA
000181955 650_7 $$2Other$$aRBM8A
000181955 650_7 $$2Other$$aRibosomal protein gene
000181955 650_7 $$2Other$$aY14
000181955 650_7 $$2Other$$aaldolase A
000181955 650_7 $$2Other$$agenetic screen
000181955 650_7 $$2Other$$agenome-wide screen
000181955 650_7 $$2Other$$aribosome biogenesis
000181955 7001_ $$aLukassen, Sören$$b1
000181955 7001_ $$aBhandare, Pranjali$$b2
000181955 7001_ $$aEing, Lorenz$$b3
000181955 7001_ $$0P:(DE-He78)7b7131e0870c28d432e48873d295460f$$aSnaebjörnsson, Marteinn Thor$$b4$$udkfz
000181955 7001_ $$aGarcía, Yiliam Cruz$$b5
000181955 7001_ $$aKisker, Jan Philipp$$b6
000181955 7001_ $$0P:(DE-He78)94ae391f53fb9285e1b68f9930615af1$$aSchulze, Almut$$b7$$udkfz
000181955 7001_ $$aWolf, Elmar$$b8
000181955 773__ $$0PERI:(DE-600)2737824-X$$a10.3389/fcell.2022.954358$$gVol. 10, p. 954358$$p954358$$tFrontiers in cell and developmental biology$$v10$$x2296-634X$$y2022
000181955 909CO $$ooai:inrepo02.dkfz.de:181955$$pVDB
000181955 9101_ $$0I:(DE-588b)2036810-0$$6P:(DE-He78)7b7131e0870c28d432e48873d295460f$$aDeutsches Krebsforschungszentrum$$b4$$kDKFZ
000181955 9101_ $$0I:(DE-588b)2036810-0$$6P:(DE-He78)94ae391f53fb9285e1b68f9930615af1$$aDeutsches Krebsforschungszentrum$$b7$$kDKFZ
000181955 9131_ $$0G:(DE-HGF)POF4-311$$1G:(DE-HGF)POF4-310$$2G:(DE-HGF)POF4-300$$3G:(DE-HGF)POF4$$4G:(DE-HGF)POF$$aDE-HGF$$bGesundheit$$lKrebsforschung$$vZellbiologie und Tumorbiologie$$x0
000181955 9141_ $$y2022
000181955 915__ $$0LIC:(DE-HGF)CCBYNV$$2V:(DE-HGF)$$aCreative Commons Attribution CC BY (No Version)$$bDOAJ$$d2021-01-30
000181955 915__ $$0StatID:(DE-HGF)0160$$2StatID$$aDBCoverage$$bEssential Science Indicators$$d2021-01-30
000181955 915__ $$0StatID:(DE-HGF)1190$$2StatID$$aDBCoverage$$bBiological Abstracts$$d2021-01-30
000181955 915__ $$0StatID:(DE-HGF)0113$$2StatID$$aWoS$$bScience Citation Index Expanded$$d2021-01-30
000181955 915__ $$0StatID:(DE-HGF)0561$$2StatID$$aArticle Processing Charges$$d2021-01-30
000181955 915__ $$0StatID:(DE-HGF)0700$$2StatID$$aFees$$d2021-01-30
000181955 915__ $$0StatID:(DE-HGF)0100$$2StatID$$aJCR$$bFRONT CELL DEV BIOL : 2021$$d2022-11-12
000181955 915__ $$0StatID:(DE-HGF)0200$$2StatID$$aDBCoverage$$bSCOPUS$$d2022-11-12
000181955 915__ $$0StatID:(DE-HGF)0300$$2StatID$$aDBCoverage$$bMedline$$d2022-11-12
000181955 915__ $$0StatID:(DE-HGF)0501$$2StatID$$aDBCoverage$$bDOAJ Seal$$d2021-05-13T10:29:32Z
000181955 915__ $$0StatID:(DE-HGF)0500$$2StatID$$aDBCoverage$$bDOAJ$$d2021-05-13T10:29:32Z
000181955 915__ $$0StatID:(DE-HGF)0030$$2StatID$$aPeer Review$$bDOAJ : Blind peer review$$d2021-05-13T10:29:32Z
000181955 915__ $$0StatID:(DE-HGF)0199$$2StatID$$aDBCoverage$$bClarivate Analytics Master Journal List$$d2022-11-12
000181955 915__ $$0StatID:(DE-HGF)0150$$2StatID$$aDBCoverage$$bWeb of Science Core Collection$$d2022-11-12
000181955 915__ $$0StatID:(DE-HGF)1050$$2StatID$$aDBCoverage$$bBIOSIS Previews$$d2022-11-12
000181955 915__ $$0StatID:(DE-HGF)9905$$2StatID$$aIF >= 5$$bFRONT CELL DEV BIOL : 2021$$d2022-11-12
000181955 9201_ $$0I:(DE-He78)A410-20160331$$kA410$$lMetabolismus und Microenvironment$$x0
000181955 980__ $$ajournal
000181955 980__ $$aVDB
000181955 980__ $$aI:(DE-He78)A410-20160331
000181955 980__ $$aUNRESTRICTED