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@ARTICLE{Schwarz:181955,
      author       = {J. D. Schwarz and S. Lukassen and P. Bhandare and L. Eing
                      and M. T. Snaebjörnsson$^*$ and Y. C. García and J. P.
                      Kisker and A. Schulze$^*$ and E. Wolf},
      title        = {{T}he glycolytic enzyme {ALDOA} and the exon junction
                      complex protein {RBM}8{A} are regulators of ribosomal
                      biogenesis.},
      journal      = {Frontiers in cell and developmental biology},
      volume       = {10},
      issn         = {2296-634X},
      address      = {Lausanne},
      publisher    = {Frontiers Media},
      reportid     = {DKFZ-2022-02325},
      pages        = {954358},
      year         = {2022},
      abstract     = {Cellular growth is a fundamental process of life and must
                      be precisely controlled in multicellular organisms. Growth
                      is crucially controlled by the number of functional
                      ribosomes available in cells. The production of new
                      ribosomes depends critically on the activity of RNA
                      polymerase (RNAP) II in addition to the activity of RNAP I
                      and III, which produce ribosomal RNAs. Indeed, the
                      expression of both, ribosomal proteins and proteins required
                      for ribosome assembly (ribosomal biogenesis factors), is
                      considered rate-limiting for ribosome synthesis. Here, we
                      used genetic screening to identify novel transcriptional
                      regulators of cell growth genes by fusing promoters from a
                      ribosomal protein gene (Rpl18) and from a ribosomal
                      biogenesis factor (Fbl) with fluorescent protein genes (RFP,
                      GFP) as reporters. Subsequently, both reporters were stably
                      integrated into immortalized mouse fibroblasts, which were
                      then transduced with a genome-wide sgRNA-CRISPR knockout
                      library. Subsequently, cells with altered reporter activity
                      were isolated by FACS and the causative sgRNAs were
                      identified. Interestingly, we identified two novel
                      regulators of growth genes. Firstly, the exon junction
                      complex protein RBM8A controls transcript levels of the
                      intronless reporters used here. By acute depletion of RBM8A
                      protein using the auxin degron system combined with the
                      genome-wide analysis of nascent transcription, we showed
                      that RBM8A is an important global regulator of ribosomal
                      protein transcripts. Secondly, we unexpectedly observed that
                      the glycolytic enzyme aldolase A (ALDOA) regulates the
                      expression of ribosomal biogenesis factors. Consistent with
                      published observations that a fraction of this protein is
                      located in the nucleus, this may be a mechanism linking
                      transcription of growth genes to metabolic processes and
                      possibly to metabolite availability.},
      keywords     = {AldoA (Other) / RBM8A (Other) / Ribosomal protein gene
                      (Other) / Y14 (Other) / aldolase A (Other) / genetic screen
                      (Other) / genome-wide screen (Other) / ribosome biogenesis
                      (Other)},
      cin          = {A410},
      ddc          = {570},
      cid          = {I:(DE-He78)A410-20160331},
      pnm          = {311 - Zellbiologie und Tumorbiologie (POF4-311)},
      pid          = {G:(DE-HGF)POF4-311},
      typ          = {PUB:(DE-HGF)16},
      pubmed       = {pmid:36187487},
      pmc          = {pmc:PMC9515781},
      doi          = {10.3389/fcell.2022.954358},
      url          = {https://inrepo02.dkfz.de/record/181955},
}