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@ARTICLE{Pervaiz:182136,
      author       = {A. Pervaiz$^*$ and T. Saleem and K. Kanwal and S. M. Raza
                      and S. Iqbal and M. Zepp$^*$ and R. Georges$^*$ and M.
                      Berger$^*$},
      title        = {{E}xpression profiling of anticancer genes in colorectal
                      cancer patients and their in vitro induction by riproximin,
                      a ribosomal inactivating plant protein.},
      journal      = {Journal of cancer research and clinical oncology},
      volume       = {149},
      number       = {8},
      issn         = {0171-5216},
      address      = {Berlin},
      publisher    = {Springer},
      reportid     = {DKFZ-2022-02453},
      pages        = {4825-4837},
      year         = {2023},
      note         = {#EA:G401#LA:G401 / 2023 Jul;149(8):4825-4837},
      abstract     = {Ectopic expression of anticancer genes (ACGs) imposes
                      antineoplastic effects on transformed cells. Clinically,
                      reduced expression of these genes has been linked with poor
                      prognosis, metastasis and chemo/radiotherapy resistance in
                      cancers. Identifying expression pattern of ACGs is crucial
                      to establish their prognostic and therapeutic relevance in
                      colorectal cancer (CRC). In addition to the clinical
                      perspective, naturally occurring compounds can be explored
                      in parallel for inducing ACGs to achieve cancer
                      cell-specific death.Expression profiles of three ACGs (NOXA,
                      PAR-4, TRAIL) were identified via real-time PCR in CRC
                      clinical isolates. Time lapse-based expression modifications
                      in ACGs were studied in a CRC liver metastasis animal model
                      using microarray methodology. Effects of a purified plant
                      protein (riproximin) on selected ACGs were identified in
                      three primary and metastatic CRC cell lines by real-time
                      PCR. Lastly, importance of the ACGs in a cellular
                      environment was highlighted via bioinformatic analysis.ACGs
                      (except NOXA) were persistently downregulated in clinical
                      isolates when comparing the overall mean expression values
                      with normal mucosa levels. In vivo studies showed a
                      prominent inhibition of NOXA and PAR-4 genes in implanted
                      CRC cells during rat liver colonization. TRAIL showed
                      deviation from this theme while showing marked induction
                      during the early period of liver colonization (days 3 and 6
                      after CRC cell implantation). Riproximin exhibited
                      substantial potential of inducing ACGs at transcriptome
                      levels in selected CRC cell lines. Bioinformatic analysis
                      showed that vital molecular/functional aspects of a cell are
                      associated with the presence of ACGs.ACGs are downregulated
                      in primary and metastatic phase of CRC. Riproximin
                      effectively induces ACGs in CRC cells and can be exploited
                      for clinical investigations over time.},
      keywords     = {Anticancer genes (Other) / Colorectal cancer (Other) /
                      Expression patterns (Other) / Inducing agent/protein (Other)
                      / Riproximin (Other)},
      cin          = {G401},
      ddc          = {610},
      cid          = {I:(DE-He78)G401-20160331},
      pnm          = {311 - Zellbiologie und Tumorbiologie (POF4-311)},
      pid          = {G:(DE-HGF)POF4-311},
      typ          = {PUB:(DE-HGF)16},
      pubmed       = {pmid:36251065},
      doi          = {10.1007/s00432-022-04410-6},
      url          = {https://inrepo02.dkfz.de/record/182136},
}