%0 Journal Article
%A Witt, Johannes
%A Haupt, Saskia
%A Ahadova, Aysel
%A Bohaumilitzky, Lena
%A Fuchs, Vera
%A Ballhausen, Alexej
%A Przybilla, Moritz Jakob
%A Jendrusch, Michael
%A Seppälä, Toni T
%A Fürst, Daniel
%A Walle, Thomas
%A Busch, Elena
%A Haag, Georg Martin
%A Hüneburg, Robert
%A Nattermann, Jacob
%A von Knebel Doeberitz, Magnus
%A Heuveline, Vincent
%A Kloor, Matthias
%T A simple approach for detecting HLA-A*02 alleles in archival formalin-fixed paraffin-embedded tissue samples and an application example for studying cancer immunoediting.
%J HLA
%V 101
%N 1
%@ 0001-2815
%C Oxford
%I Wiley
%M DKFZ-2022-02455
%P 24-33
%D 2023
%Z #EA:F210#LA:F210# / 2023 Jan;101(1):24-33
%X The human leukocyte antigen (HLA) system represents a central component of the antigen presentation machinery. As every patient possesses a defined set of HLA molecules, only certain antigens can be presented on the cell surface. Thus, studying HLA type-dependent antigen presentation can improve the understanding of variation in susceptibility to various diseases, including infectious diseases and cancer. In archival formalin-fixed paraffin-embedded (FFPE) tissue, the HLA type is difficult to analyze due to fragmentation of DNA, hindering the application of commonly used assays that rely on long DNA stretches. Addressing these difficulties, we present a refined approach for characterizing presence or absence of HLA-A*02, the most common HLA-A allele in the Caucasian population, in archival samples. We validated our genotyping strategy in a cohort of 90 samples with HLA status obtained by an NGS-based method. 90
%K Cancer immunoediting (Other)
%K Formalin-fixed paraffin-embedded tissue samples (Other)
%K HLA typing (Other)
%K MSI cancer (Other)
%F PUB:(DE-HGF)16
%9 Journal Article
%$ pmid:36251018
%R 10.1111/tan.14846
%U https://inrepo02.dkfz.de/record/182138