TY  - JOUR
AU  - Witt, Johannes
AU  - Haupt, Saskia
AU  - Ahadova, Aysel
AU  - Bohaumilitzky, Lena
AU  - Fuchs, Vera
AU  - Ballhausen, Alexej
AU  - Przybilla, Moritz Jakob
AU  - Jendrusch, Michael
AU  - Seppälä, Toni T
AU  - Fürst, Daniel
AU  - Walle, Thomas
AU  - Busch, Elena
AU  - Haag, Georg Martin
AU  - Hüneburg, Robert
AU  - Nattermann, Jacob
AU  - von Knebel Doeberitz, Magnus
AU  - Heuveline, Vincent
AU  - Kloor, Matthias
TI  - A simple approach for detecting HLA-A*02 alleles in archival formalin-fixed paraffin-embedded tissue samples and an application example for studying cancer immunoediting.
JO  - HLA
VL  - 101
IS  - 1
SN  - 0001-2815
CY  - Oxford
PB  - Wiley
M1  - DKFZ-2022-02455
SP  - 24-33
PY  - 2023
N1  - #EA:F210#LA:F210# / 2023 Jan;101(1):24-33
AB  - The human leukocyte antigen (HLA) system represents a central component of the antigen presentation machinery. As every patient possesses a defined set of HLA molecules, only certain antigens can be presented on the cell surface. Thus, studying HLA type-dependent antigen presentation can improve the understanding of variation in susceptibility to various diseases, including infectious diseases and cancer. In archival formalin-fixed paraffin-embedded (FFPE) tissue, the HLA type is difficult to analyze due to fragmentation of DNA, hindering the application of commonly used assays that rely on long DNA stretches. Addressing these difficulties, we present a refined approach for characterizing presence or absence of HLA-A*02, the most common HLA-A allele in the Caucasian population, in archival samples. We validated our genotyping strategy in a cohort of 90 samples with HLA status obtained by an NGS-based method. 90
KW  - Cancer immunoediting (Other)
KW  - Formalin-fixed paraffin-embedded tissue samples (Other)
KW  - HLA typing (Other)
KW  - MSI cancer (Other)
LB  - PUB:(DE-HGF)16
C6  - pmid:36251018
DO  - DOI:10.1111/tan.14846
UR  - https://inrepo02.dkfz.de/record/182138
ER  -