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@ARTICLE{Gatzweiler:182157,
      author       = {C. Gatzweiler$^*$ and J. Ridinger$^*$ and S. Ayhan$^*$ and
                      S. Najafi$^*$ and H. Peterziel$^*$ and O. Witt$^*$ and I.
                      Oehme$^*$},
      title        = {{E}valuation of {A}ntitumor and {O}n-{T}arget {A}ctivity of
                      {HDAC} {I}nhibitors with the {Z}ebrafish {E}mbryo
                      {X}enograft {M}odel.},
      journal      = {Methods in molecular biology},
      volume       = {2589},
      issn         = {1064-3745},
      address      = {[Heidelberg]},
      publisher    = {[Springer]},
      reportid     = {DKFZ-2022-02468},
      isbn         = {978-1-0716-2787-7 (print)},
      pages        = {75-85},
      year         = {2023},
      note         = {#EA:B310#LA:B310#},
      abstract     = {Reliable preclinical drug testing models for cancer
                      research are urgently needed with zebrafish embryo models
                      emerging as a powerful vertebrate model for
                      xenotransplantation studies. Here, we describe the
                      evaluation of toxicity, efficacy, and on-target activity of
                      histone deacetylase (HDAC) inhibitors in a zebrafish embryo
                      yolk sac xenotransplantation model of medulloblastoma and
                      neuroblastoma cells. For this, we performed toxicity assays
                      with our zebrafish drug library consisting of 28 clinically
                      relevant targeted as well as chemotherapeutic drugs with
                      zebrafish embryos. We further engrafted zebrafish embryos
                      with fluorescently labeled pediatric tumor cells
                      (SK-N-BE(2)-C, HD-MB03, or MED8A) and monitored the
                      progression after HDAC inhibitor treatment of
                      xenotransplanted tumors through tumor volume measurements
                      with high-content confocal microscopy in a multi-well
                      format. The on-target activity of HDAC inhibitors was
                      verified through immunohistochemistry staining on
                      paraffin-embedded early larvae. Overall, the zebrafish
                      embryo xenotransplantation model allows for fast and
                      cost-efficient in vivo evaluation of targeted drug toxicity,
                      efficacy, and on-target activity in the field of precision
                      oncology.},
      keywords     = {Functional precision medicine (Other) / Pediatric oncology
                      (Other) / Targeted therapy (Other) / zPDX (Other)},
      cin          = {B310 / HD01},
      ddc          = {570},
      cid          = {I:(DE-He78)B310-20160331 / I:(DE-He78)HD01-20160331},
      pnm          = {312 - Funktionelle und strukturelle Genomforschung
                      (POF4-312)},
      pid          = {G:(DE-HGF)POF4-312},
      typ          = {PUB:(DE-HGF)3 / PUB:(DE-HGF)16},
      pubmed       = {pmid:36255618},
      doi          = {10.1007/978-1-0716-2788-4_5},
      url          = {https://inrepo02.dkfz.de/record/182157},
}