TY - JOUR AU - Hübers, Corinne AU - Abdul Pari, Ashik Ahmed AU - Grieshober, Denise AU - Petkov, Martin AU - Schmidt, Alexander AU - Messmer, Tatjana AU - Heyer, Christian Moritz AU - Schölch, Sebastian AU - Kapel, Stephanie AU - Gengenbacher, Nicolas AU - Singhal, Mahak AU - Schieb, Benjamin AU - Fricke, Claudine AU - Will, Rainer AU - Remans, Kim AU - Utikal, Jochen AU - Reissfelder, Christoph AU - Schlesner, Matthias AU - Hodivala-Dilke, Kairbaan M AU - Kersten, Sander AU - Goerdt, Sergij AU - Augustin, Hellmut AU - Felcht, Moritz TI - Primary tumor-derived systemic nANGPTL4 inhibits metastasis. JO - Journal of experimental medicine VL - 220 IS - 1 SN - 0022-1007 CY - New York, NY PB - Rockefeller Univ. Press M1 - DKFZ-2022-02496 SP - e20202595 PY - 2023 N1 - #EA:A190#LA:A190# / DKFZ-ZMBH Alliance AB - Primary tumors and distant site metastases form a bidirectionally communicating system. Yet, the molecular mechanisms of this crosstalk are poorly understood. Here, we identified the proteolytically cleaved fragments of angiopoietin-like 4 (ANGPTL4) as contextually active protumorigenic and antitumorigenic contributors in this communication ecosystem. Preclinical studies in multiple tumor models revealed that the C-terminal fragment (cANGPTL4) promoted tumor growth and metastasis. In contrast, the N-terminal fragment of ANGPTL4 (nANGPTL4) inhibited metastasis and enhanced overall survival in a postsurgical metastasis model by inhibiting WNT signaling and reducing vascularity at the metastatic site. Tracing ANGPTL4 and its fragments in tumor patients detected full-length ANGPTL4 primarily in tumor tissues, whereas nANGPTL4 predominated in systemic circulation and correlated inversely with disease progression. The study highlights the spatial context of the proteolytic cleavage-dependent pro- and antitumorigenic functions of ANGPTL4 and identifies and validates nANGPTL4 as a novel biomarker of tumor progression and antimetastatic therapeutic agent. LB - PUB:(DE-HGF)16 C6 - pmid:36269299 DO - DOI:10.1084/jem.20202595 UR - https://inrepo02.dkfz.de/record/182202 ER -