Home > Publications database > The budding yeast GSK-3 homologue Mck1 is an essential component of the spindle position checkpoint. > print

001     182432
005     20240229145719.0
024 7 _ |a 10.1098/rsob.220203
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041 _ _ |a English
082 _ _ |a 570
100 1 _ |a Rathi, Siddhi
|b 0
245 _ _ |a The budding yeast GSK-3 homologue Mck1 is an essential component of the spindle position checkpoint.
260 _ _ |a London
|c 2022
|b Royal Society Publishing
336 7 _ |a article
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520 _ _ |a The spindle position checkpoint (SPOC) is a mitotic surveillance mechanism in Saccharomyces cerevisiae that prevents cells from completing mitosis in response to spindle misalignment, thereby contributing to genomic integrity. The kinase Kin4, one of the most downstream SPOC components, is essential to stop the mitotic exit network (MEN), a signalling pathway that promotes the exit from mitosis and cell division. Previous work, however, suggested that a Kin4-independent pathway contributes to SPOC, yet the underlying mechanisms remain elusive. Here, we established the glycogen-synthase-kinase-3 (GSK-3) homologue Mck1, as a novel component that works independently of Kin4 to engage SPOC. Our data indicate that both Kin4 and Mck1 work in parallel to counteract MEN activation by the Cdc14 early anaphase release (FEAR) network. We show that Mck1's function in SPOC is mediated by the pre-replication complex protein and mitotic cyclin-dependent kinase (M-Cdk) inhibitor, Cdc6, which is degraded in a Mck1-dependent manner prior to mitosis. Moderate overproduction of Cdc6 phenocopies MCK1 deletion and causes SPOC deficiency via its N-terminal, M-Cdk inhibitory domain. Our data uncover an unprecedented role of GSK-3 kinases in coordinating spindle orientation with cell cycle progression.
536 _ _ |a 311 - Zellbiologie und Tumorbiologie (POF4-311)
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650 _ 7 |a Cdc6
|2 Other
650 _ 7 |a MCK1
|2 Other
650 _ 7 |a budding yeast
|2 Other
650 _ 7 |a cell division
|2 Other
650 _ 7 |a checkpoint control
|2 Other
650 _ 7 |a mitotic exit
|2 Other
650 _ 7 |a Saccharomyces cerevisiae Proteins
|2 NLM Chemicals
650 _ 7 |a Glycogen Synthase Kinase 3
|0 EC 2.7.11.26
|2 NLM Chemicals
650 _ 7 |a Protein Serine-Threonine Kinases
|0 EC 2.7.11.1
|2 NLM Chemicals
650 _ 7 |a Cell Cycle Proteins
|2 NLM Chemicals
650 _ 7 |a CDC14 protein, S cerevisiae
|2 NLM Chemicals
650 _ 7 |a Protein Tyrosine Phosphatases
|0 EC 3.1.3.48
|2 NLM Chemicals
650 _ 7 |a MCK1 protein, S cerevisiae
|0 EC 2.7.12.1
|2 NLM Chemicals
650 _ 2 |a Humans
|2 MeSH
650 _ 2 |a Saccharomyces cerevisiae Proteins: genetics
|2 MeSH
650 _ 2 |a Glycogen Synthase Kinase 3: genetics
|2 MeSH
650 _ 2 |a Glycogen Synthase Kinase 3: metabolism
|2 MeSH
650 _ 2 |a Spindle Apparatus: metabolism
|2 MeSH
650 _ 2 |a Saccharomycetales: metabolism
|2 MeSH
650 _ 2 |a Protein Serine-Threonine Kinases
|2 MeSH
650 _ 2 |a Phosphorylation
|2 MeSH
650 _ 2 |a Saccharomyces cerevisiae: genetics
|2 MeSH
650 _ 2 |a Mitosis
|2 MeSH
650 _ 2 |a Cell Cycle Proteins: metabolism
|2 MeSH
650 _ 2 |a Protein Tyrosine Phosphatases: genetics
|2 MeSH
650 _ 2 |a Protein Tyrosine Phosphatases: metabolism
|2 MeSH
700 1 _ |a Polat, Irem
|b 1
700 1 _ |a Pereira, Gislene
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773 _ _ |a 10.1098/rsob.220203
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