%0 Journal Article
%A Meagher, Nicola S
%A Hamilton, Phineas
%A Milne, Katy
%A Thornton, Shelby
%A Harris, Bronwyn
%A Weir, Ashley
%A Alsop, Jennifer
%A Bisinoto, Christiani
%A Brenton, James D
%A Brooks-Wilson, Angela
%A Chiu, Derek S
%A Cushing-Haugen, Kara L
%A Fereday, Sian
%A Garsed, Dale W
%A Gayther, Simon A
%A Gentry-Maharaj, Aleksandra
%A Gilks, Blake
%A Jimenez-Linan, Mercedes
%A Kennedy, Catherine J
%A Le, Nhu D
%A Piskorz, Anna M
%A Riggan, Marjorie J
%A Shah, Mitul
%A Singh, Naveena
%A Talhouk, Aline
%A Widschwendter, Martin
%A Bowtell, David D L
%A Candido Dos Reis, Francisco J
%A Cook, Linda S
%A Fortner, Renée T
%A García, María J
%A Harris, Holly R
%A Huntsman, David G
%A Karnezis, Anthony N
%A Köbel, Martin
%A Menon, Usha
%A Pharoah, Paul D P
%A Doherty, Jennifer A
%A Anglesio, Michael S
%A Pike, Malcolm C
%A Pearce, Celeste Leigh
%A Friedlander, Michael L
%A DeFazio, Anna
%A Nelson, Brad H
%A Ramus, Susan J
%T Profiling the immune landscape in mucinous ovarian carcinoma.
%J Gynecologic oncology
%V 168
%@ 0090-8258
%C Amsterdam [u.a.]
%I Elsevier
%M DKFZ-2022-02736
%P 23 - 31
%D 2022
%X Mucinous ovarian carcinoma (MOC) is a rare histotype of ovarian cancer, with low response rates to standard chemotherapy, and very poor survival for patients diagnosed at advanced stage. There is a limited understanding of the MOC immune landscape, and consequently whether immune checkpoint inhibitors could be considered for a subset of patients.We performed multicolor immunohistochemistry (IHC) and immunofluorescence (IF) on tissue microarrays in a cohort of 126 MOC patients. Cell densities were calculated in the epithelial and stromal components for tumor-associated macrophages (CD68+/PD-L1+, CD68+/PD-L1-), T cells (CD3+/CD8-, CD3+/CD8+), putative T-regulatory cells (Tregs, FOXP3+), B cells (CD20+/CD79A+), plasma cells (CD20-/CD79a+), and PD-L1+ and PD-1+ cells, and compared these values with clinical factors. Univariate and multivariable Cox Proportional Hazards assessed overall survival. Unsupervised k-means clustering identified patient subsets with common patterns of immune cell infiltration.Mean densities of PD1+ cells, PD-L1- macrophages, CD4+ and CD8+ T cells, and FOXP3+ Tregs were higher in the stroma compared to the epithelium. Tumors from advanced (Stage III/IV) MOC had greater epithelial infiltration of PD-L1- macrophages, and fewer PD-L1+ macrophages compared with Stage I/II cancers (p = 0.004 and p = 0.014 respectively). Patients with high epithelial density of FOXP3+ cells, CD8+/FOXP3+ cells, or PD-L1- macrophages, had poorer survival, and high epithelial CD79a + plasma cells conferred better survival, all upon univariate analysis only. Clustering showed that most MOC (86
%K Immune infiltrate (Other)
%K Mucinous ovarian carcinoma (Other)
%K Rare histotype (Other)
%F PUB:(DE-HGF)16
%9 Journal Article
%$ pmid:36368129
%R 10.1016/j.ygyno.2022.10.022
%U https://inrepo02.dkfz.de/record/182555