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000182685 0247_ $$2doi$$a10.3389/fimmu.2022.1040031
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000182685 041__ $$aEnglish
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000182685 1001_ $$aKurt, Ada S$$b0
000182685 245__ $$aIL-2 availability regulates the tissue specific phenotype of murine intra-hepatic Tregs.
000182685 260__ $$aLausanne$$bFrontiers Media$$c2022
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000182685 520__ $$aCD4+CD25+Foxp3+ Tregs are known to acquire tissue-specific features and exert cytoprotective and regenerative functions. The extent to which this applies to liver-resident Tregs is unknown. In this study, we aimed to explore the phenotypic and functional characteristics of adult murine liver resident Tregs during homeostasis. Additionally, we investigated their role in ameliorating liver inflammation and tissue damage. Quantification of Foxp3+CD4+CD25+ cells comparing different tissues showed that the liver contained significantly fewer resident Tregs. A combination of flow cytometry phenotyping and microarray analysis of intra-hepatic and splenic Tregs under homeostatic conditions revealed that, although intra-hepatic Tregs exhibited the core transcriptional Treg signature, they expressed a distinct transcriptional profile. This was characterized by reduced CD25 expression and increased levels of pro-inflammatory Th1 transcripts Il1b and Ifng. In vivo ablation of Tregs in the Foxp3-DTR mouse model showed that Tregs had a role in reducing the magnitude of systemic and intra-hepatic inflammatory responses following acute carbon tetrachloride (CCl₄) injury, but their absence did not impact the development of hepatocyte necrosis. Conversely, the specific expansion of Tregs by administration of IL-2 complexes increased the number of intra-hepatic Tregs and significantly ameliorated tissue damage following CCl₄ administration in C57BL/6 mice. The cytoprotective effect observed in response to IL-2c was associated with the increased expression of markers known to regulate Treg suppressive function. Our results offer insight into the transcriptome and complex immune network of intra-hepatic Tregs and suggest that strategies capable of selectively increasing the pool of intra-hepatic Tregs could constitute effective therapies in inflammatory liver diseases.
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000182685 650_7 $$2Other$$aCCl₄ induced liver injury
000182685 650_7 $$2Other$$aTreg depletion
000182685 650_7 $$2Other$$aacute inflammation
000182685 650_7 $$2Other$$aliver
000182685 650_7 $$2Other$$aregulatory T cells (Tregs)
000182685 650_7 $$2Other$$atissue specific
000182685 7001_ $$aStrobl, Karoline$$b1
000182685 7001_ $$aRuiz, Paula$$b2
000182685 7001_ $$aOsborn, Gabriel$$b3
000182685 7001_ $$aChester, Tonika$$b4
000182685 7001_ $$aDawson, Lauren$$b5
000182685 7001_ $$0P:(DE-He78)ae4deeee80cae8c94cc144333ae4cc5a$$aWarwas, Karsten M$$b6$$udkfz
000182685 7001_ $$aGrey, Elizabeth H$$b7
000182685 7001_ $$aMastoridis, Sotiris$$b8
000182685 7001_ $$aKodela, Elisavet$$b9
000182685 7001_ $$aSafinia, Niloufar$$b10
000182685 7001_ $$aSanchez-Fueyo, Alberto$$b11
000182685 7001_ $$aMartinez-Llordella, Marc$$b12
000182685 773__ $$0PERI:(DE-600)2606827-8$$a10.3389/fimmu.2022.1040031$$gVol. 13, p. 1040031$$p1040031$$tFrontiers in immunology$$v13$$x1664-3224$$y2022
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