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@ARTICLE{Li:182780,
      author       = {A. Li and S. Muenst and J. Hoffman and L. Starck and M.
                      Sarem and A. Fischer$^*$ and G. Hutter and V. P. Shastri},
      title        = {{M}esenchymal-endothelial nexus in breast cancer spheroids
                      induces vasculogenesis and local invasion in a {CAM} model.},
      journal      = {Communications biology},
      volume       = {5},
      number       = {1},
      issn         = {2399-3642},
      address      = {London},
      publisher    = {Springer Nature},
      reportid     = {DKFZ-2022-02912},
      pages        = {1303},
      year         = {2022},
      abstract     = {Interplay between non-cancerous cells (immune, fibroblasts,
                      mesenchymal stromal cells (MSC), and endothelial cells (EC))
                      has been identified as vital in driving tumor progression.
                      As studying such interactions in vivo is challenging, ex
                      vivo systems that can recapitulate in vivo scenarios can aid
                      in unraveling the factors impacting tumorigenesis and
                      metastasis. Using the synthetic tumor microenvironment
                      mimics (STEMs)-a spheroid system composed of breast cancer
                      cells (BCC) with defined human MSC and EC fractions, here we
                      show that EC organization into vascular structures is BC
                      phenotype dependent, and independent of ERα expression in
                      epithelial cancer cells, and involves MSC-mediated Notch1
                      signaling. In a 3D-bioprinted model system to mimic local
                      invasion, MDA STEMs collectively respond to serum gradient
                      and form invading cell clusters. STEMs grown on chick
                      chorioallantoic membrane undergo local invasion to form CAM
                      tumors that can anastomose with host vasculature and bear
                      the typical hallmarks of human BC and this process requires
                      both EC and MSC. This study provides a framework for
                      developing well-defined in vitro systems, including
                      patient-derived xenografts that recapitulate in vivo events,
                      to investigate heterotypic cell interactions in tumors, to
                      identify factors promoting tumor metastasis-related events,
                      and possibly drug screening in the context of personalized
                      medicine.},
      cin          = {A270},
      ddc          = {570},
      cid          = {I:(DE-He78)A270-20160331},
      pnm          = {311 - Zellbiologie und Tumorbiologie (POF4-311)},
      pid          = {G:(DE-HGF)POF4-311},
      typ          = {PUB:(DE-HGF)16},
      pubmed       = {pmid:36435836},
      doi          = {10.1038/s42003-022-04236-5},
      url          = {https://inrepo02.dkfz.de/record/182780},
}