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@ARTICLE{Vit:186337,
author = {G. Vit$^*$ and A. Hirth$^*$ and N. Neugebauer$^*$ and B. N.
Kraft$^*$ and G. Sigismondo$^*$ and A. Cazzola$^*$ and C.
Tessmer$^*$ and J. Duro and J. Krijgsveld$^*$ and I.
Hofmann$^*$ and M. Berger and H. Klüter and C. Niehrs$^*$
and J. Nilsson and A. Krämer$^*$},
title = {{H}uman {SLFN}5 and its {X}enopus {L}aevis ortholog
regulate entry into mitosis and oocyte meiotic resumption.},
journal = {Cell death discovery},
volume = {8},
number = {1},
issn = {2058-7716},
address = {London},
publisher = {Nature Publishing Group},
reportid = {DKFZ-2022-03105},
pages = {484},
year = {2022},
note = {#EA:A360#LA:A360# / DKFZ-ZMBH Alliance},
abstract = {The Schlafen gene family was first described in mice as a
regulator of thymocyte development. Further studies showed
involvement of human orthologs in different processes
related with viral replication, cellular proliferation, and
differentiation. In recent years, a new role for human
Slfn11 in DNA replication and chromatin remodeling was
described. As commonly observed in many gene families, Slfn
paralogs show a tissue-specific expression. This made it
difficult to reach conclusions which can be valid in
different biological models regarding the function of the
different Schlafen proteins. In the present study, we
investigate the involvement of SLFN5 in cell-cycle
regulation and cell proliferation. A careful analysis of
SLFN5 expression revealed that SLFN5 is highly expressed in
proliferating tissues and that the protein is ubiquitously
present in all the tissues and cell line models we analyzed.
Very interestingly, SLFN5 expression oscillates during cell
cycle, peaking during S phase. The fact that SLFN5 interacts
with protein phosphatase 2A and that SLFN5 depletion causes
cell cycle arrest and cellular apoptosis, suggests a direct
involvement of this human paralog in cell cycle progression
and cellular proliferation. We substantiated our in vitro
and in cellulo results using Xenopus laevis oocytes to show
that mRNA depletion of the unique Slfn gene present in
Xenopus, whose protein sequence shares $80\%$ of homology
with SLFN5, recapitulates the phenotype observed in human
cells preventing the resumption of meiosis during oocyte
development.},
cin = {A360 / A050 / B230 / W170},
ddc = {610},
cid = {I:(DE-He78)A360-20160331 / I:(DE-He78)A050-20160331 /
I:(DE-He78)B230-20160331 / I:(DE-He78)W170-20160331},
pnm = {311 - Zellbiologie und Tumorbiologie (POF4-311)},
pid = {G:(DE-HGF)POF4-311},
typ = {PUB:(DE-HGF)16},
pubmed = {pmid:36477080},
pmc = {pmc:PMC9729291},
doi = {10.1038/s41420-022-01274-0},
url = {https://inrepo02.dkfz.de/record/186337},
}