TY  - JOUR
AU  - Caydasi, Ayse Koca
AU  - Khmelinskii, Anton
AU  - Darieva, Zoulfia
AU  - Kurtulmus, Bahtiyar
AU  - Knop, Michael
AU  - Pereira, Gislene
TI  - SWR1 Chromatin Remodeling Complex Prevents Mitotic Slippage during Spindle Position Checkpoint Arrest.
JO  - Molecular biology of the cell
VL  - 34
IS  - 2
SN  - 1059-1524
CY  - Bethesda, Md.
PB  - American Society for Cell Biology
M1  - DKFZ-2022-03166
SP  - ar11b
PY  - 2023
N1  - DKFZ-ZMBH Alliance / 2023 Feb 1;34(2):ar11b / #LA:A180#
AB  - Faithful chromosome segregation in budding yeast requires correct positioning of the mitotic spindle along the mother to daughter cell polarity axis. When the anaphase spindle is not correctly positioned, a surveillance mechanism, named as the spindle position checkpoint (SPOC), prevents the progression out of mitosis until correct spindle positioning is achieved. How SPOC works on a molecular level is not well understood. Here, we performed a genome-wide genetic screen to search for components required for SPOC. We identified the SWR1 chromatin-remodeling complex (SWR1-C) among several novel factors that are essential for SPOC integrity. Cells lacking SWR1-C were able to activate SPOC upon spindle misorientation but underwent mitotic slippage upon prolonged SPOC arrest. This mitotic slippage required the Cdc14-early anaphase release pathway and other factors including the SAGA histone acetyltransferase complex, proteasome components and the mitotic cyclin-dependent kinase inhibitor Sic1. Together, our data establish a novel link between SWR1-C chromatin remodeling and robust checkpoint arrest in late anaphase.
LB  - PUB:(DE-HGF)16
C6  - pmid:36542480
DO  - DOI:10.1091/mbc.E20-03-0179
UR  - https://inrepo02.dkfz.de/record/186399
ER  -