% IMPORTANT: The following is UTF-8 encoded. This means that in the presence % of non-ASCII characters, it will not work with BibTeX 0.99 or older. % Instead, you should use an up-to-date BibTeX implementation like “bibtex8” or % “biber”. @ARTICLE{Trochopoulos:186458, author = {A. G. X. Trochopoulos and Y. Ilieva and A. D. Kroumov and L. L. Dimitrova and I. Pencheva-El Tibi and S. Philipov and M. Berger$^*$ and H. M. Najdenski and K. Yoncheva and S. M. Konstantinov and M. M. Zaharieva}, title = {{M}icellar {C}urcumin {S}ubstantially {I}ncreases the {A}ntineoplastic {A}ctivity of the {A}lkylphosphocholine {E}rufosine against {TWIST}1 {P}ositive {C}utaneous {T} {C}ell {L}ymphoma {C}ell {L}ines.}, journal = {Pharmaceutics}, volume = {14}, number = {12}, issn = {1999-4923}, address = {Basel}, publisher = {MDPI}, reportid = {DKFZ-2022-03179}, pages = {2688}, year = {2022}, abstract = {Cutaneous T-cell lymphoma (CTCL) is a rare form of cancer with local as well as systemic manifestations. Concomitant bacterial infections increase morbidity and mortality rates due to impaired skin barrier and immune deficiency. In the current study, we demonstrated that the in vitro anti-lymphoma potential of erufosine is diminished by TWIST1 expression and micellar curcumin substantially increases its antineoplastic activity. Pharmacokinetic analysis showed that the micellar curcumin (MCRM) used in our study was characterized by low zeta potential, slow release of curcumin, and fast cell membrane penetration. The combination ratio 1:4 [erufosine:MCRM] achieved strong synergism by inhibiting cell proliferation and clonogenicity. The combined antiproliferative effects were calculated using the symbolic mathematical software MAPLE 15. The synergistic combination strongly decreased the expression of TWIST1 and protein kinase B/Akt as proven by western blotting. Significant reductions in NF-κB activation, induction of apoptosis, and altered glutathione levels were demonstrated by corresponding assays. In addition, the synergistic combination enhanced the anti-staphylococcal activity and prevented biofilm formation, as shown by crystal violet staining. Taken together, the above results show that the development of nanotechnological treatment modalities for CTCL, based on rational drug combinations exhibiting parallel antineoplastic and antibacterial effects, may prove efficacious.}, keywords = {TWIST1 (Other) / curcumin (Other) / cutaneous T-cell lymphoma (Other) / erufosine (Other) / nanotechnology (Other) / synergy (Other)}, cin = {G401}, ddc = {610}, cid = {I:(DE-He78)G401-20160331}, pnm = {311 - Zellbiologie und Tumorbiologie (POF4-311)}, pid = {G:(DE-HGF)POF4-311}, typ = {PUB:(DE-HGF)16}, pubmed = {pmid:36559182}, pmc = {pmc:PMC9781439}, doi = {10.3390/pharmaceutics14122688}, url = {https://inrepo02.dkfz.de/record/186458}, }