% IMPORTANT: The following is UTF-8 encoded. This means that in the presence
% of non-ASCII characters, it will not work with BibTeX 0.99 or older.
% Instead, you should use an up-to-date BibTeX implementation like “bibtex8” or
% “biber”.
@ARTICLE{Schllhorn:265700,
author = {A. Schöllhorn and A. Maia and F. Kimmerle and J. Born and
H.-G. Rammensee$^*$ and S. Dimitrov and C.
Gouttefangeas$^*$},
title = {{S}taining of activated ß2-integrins in combination with
{CD}137 and {CD}154 for sensitive identification of
functional antigen-specific {CD}4+ and {CD}8+ {T} cells.},
journal = {Frontiers in immunology},
volume = {13},
issn = {1664-3224},
address = {Lausanne},
publisher = {Frontiers Media},
reportid = {DKFZ-2023-00295},
pages = {1107366},
year = {2023},
abstract = {Common flow cytometry-based methods used for functional
assessment of antigen-specific T cells rely on de novo
expression of intracellular cytokines or cell surface
activation induced markers. They come with some limitations
such as complex experimental setting, loss of cell viability
and often high unspecific background which impairs assay
sensitivity. We have previously shown that staining of
activated ß2-integrins either with multimers of their
ligand ICAM-1 or with a monoclonal antibody can serve as a
functional marker detectable on T cells after minutes (CD8+)
or few hours (CD4+) of activation. Here, we present a simple
method for detection of activated ß2-integrins in
combination with established cell surface activation induced
markers. We observed that activated ß2-integrins were still
detectable after 14 hours of stimulation, allowing their
detection together with CD137 and CD154. Combinatorial
gating of cells expressing activated ß2-integrins and CD137
or CD154 reduced background in unstimulated samples,
increasing the signal-to-noise ratio and allowing improved
assessment of low-frequency T cell responses. Extracellular
staining of these markers highly correlated with production
of intracellular cytokines IL-2, TNF or IFNγ in CD4+ and
CD8+ T cells. As an exemplary application, SARS-CoV-2
spike-specific T cell responses were assessed in individuals
after COVID-19 vaccination. This method should be useful for
epitope discovery projects and for the simultaneous
monitoring of low-frequency antigen-specific CD4+ and CD8+ T
cell responses in various physiological situations.},
keywords = {Humans / CD8-Positive T-Lymphocytes / CD4-Positive
T-Lymphocytes / Integrins: metabolism / COVID-19 Vaccines:
metabolism / COVID-19: metabolism / SARS-CoV-2 / Antigens:
metabolism / CD40 Ligand / Cytokines: metabolism / CD137
(Other) / CD154 (Other) / CD4+ T cells (Other) / CD8+ T
cells (Other) / activation induced marker (AIM) (Other) /
antigen-specificity (Other) / cell surface staining (Other)
/ integrin activation (Other) / Integrins (NLM Chemicals) /
COVID-19 Vaccines (NLM Chemicals) / Antigens (NLM Chemicals)
/ CD40 Ligand (NLM Chemicals) / Cytokines (NLM Chemicals)},
cin = {TU01},
ddc = {610},
cid = {I:(DE-He78)TU01-20160331},
pnm = {899 - ohne Topic (POF4-899)},
pid = {G:(DE-HGF)POF4-899},
typ = {PUB:(DE-HGF)16},
pubmed = {pmid:36741378},
pmc = {pmc:PMC9892897},
doi = {10.3389/fimmu.2022.1107366},
url = {https://inrepo02.dkfz.de/record/265700},
}