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@ARTICLE{Peiffer:267543,
      author       = {L. Peiffer$^*$ and T. Gambichler and T. B. Buus and K.
                      Horny$^*$ and J. Gravemeyer$^*$ and F. Furtmann and I.
                      Spassova$^*$ and L. Kubat$^*$ and L. Susok and R.
                      Stranzenbach and N. Srinivas$^*$ and N. Ødum and J. C.
                      Becker$^*$},
      title        = {{P}henotypic plasticity of malignant {T} cells in blood and
                      skin of a {S}ézary syndrome patient revealed by single cell
                      transcriptomics.},
      journal      = {Frontiers in oncology},
      volume       = {13},
      issn         = {2234-943X},
      address      = {Lausanne},
      publisher    = {Frontiers Media},
      reportid     = {DKFZ-2023-00321},
      pages        = {1090592},
      year         = {2023},
      abstract     = {Sézary Syndrome (SS) is an aggressive leukemic variant of
                      cutaneous T-cell lymphomas (CTCL). In SS patients, malignant
                      T cells are circulating through the blood and cause
                      erythroderma.To compare the transcriptome of single cells in
                      blood and skin samples from a patient with advanced SS.We
                      utilized combined single cell RNA and T-cell receptor (TCR)
                      sequencing (scRNA-seq).We scrutinized the malignant T cells
                      in blood and skin in an unbiased manner without pre-sorting
                      of cells. We observed different phenotypes of the same
                      monoclonal malignant T-cell population, confirmed by TCR
                      sequencing and inferred copy number variation analysis.
                      Malignant T cells present in the circulating blood expressed
                      genes resembling central memory T cells such as CCR7, IL7R
                      and CD27. In the skin, we detected two major malignant
                      T-cell populations: One subpopulation was closely related to
                      the malignant T cells from the blood, while the other
                      subpopulation expressed genes reminiscent of skin resident
                      effector memory T cells including GZMB and NKG7. Pseudotime
                      analysis indicated crucial transcriptomic changes in the
                      transition of malignant T cells between blood and skin.
                      These changes included the differential regulation of TXNIP,
                      a putative tumor suppressor in CTCL, and the adaptation to
                      the hypoxic conditions in the skin. Tumor cell proliferation
                      in the skin was supported by stimulating interactions
                      between myeloid cells and malignant T cells.Using scRNA-seq
                      we detected a high degree of functional heterogeneity within
                      the malignant T-cell population in SS and highlighted
                      crucial differences between SS cells in blood and skin.},
      keywords     = {Sézary syndrome (Other) / cutaneous T cell lymphoma
                      (Other) / inflammation (Other) / malignant T cells (Other) /
                      reactive T cells (Other) / scRNAseq (Other)},
      cin          = {ED01},
      ddc          = {610},
      cid          = {I:(DE-He78)ED01-20160331},
      pnm          = {899 - ohne Topic (POF4-899)},
      pid          = {G:(DE-HGF)POF4-899},
      typ          = {PUB:(DE-HGF)16},
      pubmed       = {pmid:36761972},
      pmc          = {pmc:PMC9905421},
      doi          = {10.3389/fonc.2023.1090592},
      url          = {https://inrepo02.dkfz.de/record/267543},
}