TY - JOUR AU - Nikitina, Ekaterina AU - Burk-Körner, Amelie AU - Wiesenfarth, Manuel AU - Alwers, Elizabeth AU - Heide, Danijela AU - Tessmer, Claudia AU - Ernst, Claudia AU - Krunic, Damir AU - Schrotz-King, Petra AU - Chang-Claude, Jenny AU - von Winterfeld, Moritz AU - Herpel, Esther AU - Brobeil, Alexander AU - Brenner, Hermann AU - Heikenwälder, Mathias AU - Hoffmeister, Michael AU - Kopp-Schneider, Annette AU - Bund, Timo TI - Bovine meat and milk factor protein expression in tumor-free mucosa of colorectal cancer patients coincides with macrophages and might interfere with patient survival. JO - Molecular oncology VL - 18 IS - 5 SN - 1574-7891 CY - Hoboken, NJ PB - John Wiley & Sons, Inc. M1 - DKFZ-2023-00385 SP - 1076-1092 PY - 2024 N1 - #EA:F200#LA:F200# / 2024 May;18(5):1076-1092 AB - Bovine milk and meat factors (BMMFs) are plasmid-like DNA molecules isolated from bovine milk and serum, as well as the peritumor of colorectal cancer (CRC) patients. BMMFs have been proposed as zoonotic infectious agents and drivers of indirect carcinogenesis of CRC, inducing chronic tissue inflammation, radical formation and increased levels of DNA damage. Data on expression of BMMFs in large clinical cohorts to test an association with co-markers and clinical parameters were not previously available and were therefore assessed in this study. Tissue sections with paired tumor-adjacent mucosa and tumor tissues of CRC patients [individual cohorts and tissue microarrays (TMAs) (n = 246)], low-/high-grade dysplasia (LGD/HGD) and mucosa of healthy donors were used for immunohistochemical quantification of the expression of BMMF replication protein (Rep) and CD68/CD163 (macrophages) by co-immunofluorescence microscopy and immunohistochemical scoring (TMA). Rep was expressed in the tumor-adjacent mucosa of 99 KW - bovine meat and milk factors (Other) KW - chronic inflammation (Other) KW - colorectal cancer (Other) KW - infectious indirect carcinogenesis (Other) LB - PUB:(DE-HGF)16 C6 - pmid:36811271 DO - DOI:10.1002/1878-0261.13390 UR - https://inrepo02.dkfz.de/record/271253 ER -