TY  - JOUR
AU  - Obermayer, Benedikt
AU  - Keilholz, Luisa
AU  - Conrad, Thomas
AU  - Frentsch, Marco
AU  - Blau, Igor-Wolfgang
AU  - Vuong, Lam
AU  - Lesch, Stella
AU  - Movasshagi, Kamran
AU  - Tietze-Stolley, Carola
AU  - Loyal, Lucie
AU  - Henze, Larissa
AU  - Penack, Olaf
AU  - Stervbo, Ulrik
AU  - Babel, Nina
AU  - Haas, Simon
AU  - Beule, Dieter
AU  - Bullinger, Lars
AU  - Wittenbecher, Friedrich
AU  - Na, Il-Kang
TI  - Single-cell clonal tracking of persistent T-cells in allogeneic hematopoietic stem cell transplantation.
JO  - Frontiers in immunology
VL  - 14
SN  - 1664-3224
CY  - Lausanne
PB  - Frontiers Media
M1  - DKFZ-2023-00429
SP  - 1114368
PY  - 2023
AB  - The critical balance between intended and adverse effects in allogeneic hematopoietic stem cell transplantation (alloHSCT) depends on the fate of individual donor T-cells. To this end, we tracked αβT-cell clonotypes during stem cell mobilization treatment with granulocyte-colony stimulating factor (G-CSF) in healthy donors and for six months during immune reconstitution after transfer to transplant recipients. More than 250 αβT-cell clonotypes were tracked from donor to recipient. These clonotypes consisted almost exclusively of CD8+ effector memory T cells (CD8TEM), which exhibited a different transcriptional signature with enhanced effector and cytotoxic functions compared to other CD8TEM. Importantly, these distinct and persisting clonotypes could already be delineated in the donor. We confirmed these phenotypes on the protein level and their potential for selection from the graft. Thus, we identified a transcriptional signature associated with persistence and expansion of donor T-cell clonotypes after alloHSCT that may be exploited for personalized graft manipulation strategies in future studies.
KW  - T-cells (Other)
KW  - allogeneic HSCT (Other)
KW  - clonal tracking (Other)
KW  - leukemia (Other)
KW  - scRNAseq (Other)
KW  - transplantation (Other)
LB  - PUB:(DE-HGF)16
C6  - pmid:36860867
C2  - pmc:PMC9969884
DO  - DOI:10.3389/fimmu.2023.1114368
UR  - https://inrepo02.dkfz.de/record/274083
ER  -