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@ARTICLE{Obermayer:274083,
      author       = {B. Obermayer and L. Keilholz and T. Conrad and M. Frentsch
                      and I.-W. Blau and L. Vuong and S. Lesch and K. Movasshagi
                      and C. Tietze-Stolley and L. Loyal and L. Henze and O.
                      Penack and U. Stervbo and N. Babel and S. Haas$^*$ and D.
                      Beule and L. Bullinger$^*$ and F. Wittenbecher and I.-K.
                      Na$^*$},
      title        = {{S}ingle-cell clonal tracking of persistent {T}-cells in
                      allogeneic hematopoietic stem cell transplantation.},
      journal      = {Frontiers in immunology},
      volume       = {14},
      issn         = {1664-3224},
      address      = {Lausanne},
      publisher    = {Frontiers Media},
      reportid     = {DKFZ-2023-00429},
      pages        = {1114368},
      year         = {2023},
      abstract     = {The critical balance between intended and adverse effects
                      in allogeneic hematopoietic stem cell transplantation
                      (alloHSCT) depends on the fate of individual donor T-cells.
                      To this end, we tracked αβT-cell clonotypes during stem
                      cell mobilization treatment with granulocyte-colony
                      stimulating factor (G-CSF) in healthy donors and for six
                      months during immune reconstitution after transfer to
                      transplant recipients. More than 250 αβT-cell clonotypes
                      were tracked from donor to recipient. These clonotypes
                      consisted almost exclusively of CD8+ effector memory T cells
                      (CD8TEM), which exhibited a different transcriptional
                      signature with enhanced effector and cytotoxic functions
                      compared to other CD8TEM. Importantly, these distinct and
                      persisting clonotypes could already be delineated in the
                      donor. We confirmed these phenotypes on the protein level
                      and their potential for selection from the graft. Thus, we
                      identified a transcriptional signature associated with
                      persistence and expansion of donor T-cell clonotypes after
                      alloHSCT that may be exploited for personalized graft
                      manipulation strategies in future studies.},
      keywords     = {T-cells (Other) / allogeneic HSCT (Other) / clonal tracking
                      (Other) / leukemia (Other) / scRNAseq (Other) /
                      transplantation (Other)},
      cin          = {BE01},
      ddc          = {610},
      cid          = {I:(DE-He78)BE01-20160331},
      pnm          = {899 - ohne Topic (POF4-899)},
      pid          = {G:(DE-HGF)POF4-899},
      typ          = {PUB:(DE-HGF)16},
      pubmed       = {pmid:36860867},
      pmc          = {pmc:PMC9969884},
      doi          = {10.3389/fimmu.2023.1114368},
      url          = {https://inrepo02.dkfz.de/record/274083},
}