%0 Journal Article
%A Molostvov, Guerman
%A Gachechiladze, Mariam
%A Shaaban, Abeer M
%A Hayward, Steven
%A Dean, Isaac
%A Dias, Irundika H K
%A Badr, Nahla
%A Danial, Irini
%A Mohammed, Fiyaz
%A Novitskaya, Vera
%A Paniushkina, Liliia
%A Speirs, Valerie
%A Hanby, Andrew
%A Nazarenko, Irina
%A Withers, David R
%A van Laere, Steven
%A Long, Heather M
%A Berditchevski, Fedor
%T Tspan6 stimulates the chemoattractive potential of breast cancer cells for B cells in an EV- and LXR-dependent manner.
%J Cell reports
%V 42
%N 3
%@ 2211-1247
%C [New York, NY]
%I Elsevier
%M DKFZ-2023-00444
%P 112207
%D 2023
%X The immune microenvironment in breast cancer (BCa) is controlled by a complex network of communication between various cell types. Here, we find that recruitment of B lymphocytes to BCa tissues is controlled via mechanisms associated with cancer cell-derived extracellular vesicles (CCD-EVs). Gene expression profiling identifies the Liver X receptor (LXR)-dependent transcriptional network as a key pathway that controls both CCD-EVs-induced migration of B cells and accumulation of B cells in BCa tissues. The increased accumulation oxysterol ligands for LXR (i.e., 25-hydroxycholesterol and 27-hydroxycholesterol) in CCD-EVs is regulated by the tetraspanin 6 (Tspan6). Tspan6 stimulates the chemoattractive potential of BCa cells for B cells in an EV- and LXR-dependent manner. These results demonstrate that tetraspanins control intercellular trafficking of oxysterols via CCD-EVs. Furthermore, tetraspanin-dependent changes in the oxysterol composition of CCD-EVs and the LXR signaling axis play a key role in specific changes in the tumor immune microenvironment.
%K B cells (Other)
%K CP: Cancer (Other)
%K LXR (Other)
%K breast cancer (Other)
%K oxysterols (Other)
%K tetraspanins (Other)
%K tumor microenvironment (Other)
%F PUB:(DE-HGF)16
%9 Journal Article
%$ pmid:36867531
%R 10.1016/j.celrep.2023.112207
%U https://inrepo02.dkfz.de/record/274134