TY  - JOUR
AU  - Untergasser, Andreas
AU  - Hellemans, Jan
AU  - Pfaffl, Michael W
AU  - Ruijter, Jan M
AU  - van den Hoff, Maurice J B
AU  - Dragomir, Mihnea-Paul
AU  - Adamoski, Douglas
AU  - Dias, Sandra Martha Gomes
AU  - Reis, Rui Manuel
AU  - Ferracin, Manuela
AU  - Dias-Neto, Emmanuel
AU  - Marsh, Ian
AU  - Kubista, Mikael
AU  - Fabbri, Muller
AU  - Goel, Ajay
AU  - Slabý, Ondřej
AU  - Knutsen, Erik
AU  - Chen, BaoQing
AU  - Negrini, Massimo
AU  - Mimori, Koshi
AU  - Pichler, Martin
AU  - Papatriantafyllou, Maria
AU  - Anfossi, Simone
AU  - Schmittgen, Thomas D
AU  - Huggett, Jim
AU  - Bustin, Stephen
AU  - Vandesompele, Jo
AU  - Calin, George A
TI  - Disclosing quantitative RT-PCR raw data during manuscript submission:a call for action.
JO  - Molecular oncology
VL  - 17
IS  - 5
SN  - 1574-7891
CY  - Hoboken, NJ
PB  - John Wiley & Sons, Inc.
M1  - DKFZ-2023-00530
SP  - 713-717
PY  - 2023
N1  - 2023 May;17(5):713-717
AB  - Accuracy and transparency of scientific data are becoming more and more relevant with the increasing concern regarding the evaluation of data reproducibility in many research areas. This concern is also true for quantifying coding and non-coding RNAs, with the remarkable increase in publications reporting RNA profiling and sequencing studies. To address the problem, we propose the following recommendations: 1) accurate documentation of experimental procedures in Materials and Methods (and not only in the supplementary information, as many journals have a strict mandate for making Materials and Methods as visible as possible in the main text); 2) submission of RT-qPCR raw data for all experiments reported; and 3) adoption of a unified, simple format for submitted RT-qPCR raw data. The Real-time PCR Data Essential Spreadsheet Format (RDES) was created for this purpose.
KW  - Accuracy (Other)
KW  - RNA (Other)
KW  - RT-qPCR (Other)
KW  - quantification (Other)
LB  - PUB:(DE-HGF)16
C6  - pmid:36916500
DO  - DOI:10.1002/1878-0261.13418
UR  - https://inrepo02.dkfz.de/record/274310
ER  -