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@ARTICLE{Untergasser:274310,
      author       = {A. Untergasser and J. Hellemans and M. W. Pfaffl and J. M.
                      Ruijter and M. J. B. van den Hoff and M.-P. Dragomir$^*$ and
                      D. Adamoski and S. M. G. Dias and R. M. Reis and M. Ferracin
                      and E. Dias-Neto and I. Marsh and M. Kubista and M. Fabbri
                      and A. Goel and O. Slabý and E. Knutsen and B. Chen and M.
                      Negrini and K. Mimori and M. Pichler and M.
                      Papatriantafyllou and S. Anfossi and T. D. Schmittgen and J.
                      Huggett and S. Bustin and J. Vandesompele and G. A. Calin},
      collaboration = {HEROIC},
      title        = {{D}isclosing quantitative {RT}-{PCR} raw data during
                      manuscript submission:a call for action.},
      journal      = {Molecular oncology},
      volume       = {17},
      number       = {5},
      issn         = {1574-7891},
      address      = {Hoboken, NJ},
      publisher    = {John Wiley $\&$ Sons, Inc.},
      reportid     = {DKFZ-2023-00530},
      pages        = {713-717},
      year         = {2023},
      note         = {2023 May;17(5):713-717},
      abstract     = {Accuracy and transparency of scientific data are becoming
                      more and more relevant with the increasing concern regarding
                      the evaluation of data reproducibility in many research
                      areas. This concern is also true for quantifying coding and
                      non-coding RNAs, with the remarkable increase in
                      publications reporting RNA profiling and sequencing studies.
                      To address the problem, we propose the following
                      recommendations: 1) accurate documentation of experimental
                      procedures in Materials and Methods (and not only in the
                      supplementary information, as many journals have a strict
                      mandate for making Materials and Methods as visible as
                      possible in the main text); 2) submission of RT-qPCR raw
                      data for all experiments reported; and 3) adoption of a
                      unified, simple format for submitted RT-qPCR raw data. The
                      Real-time PCR Data Essential Spreadsheet Format (RDES) was
                      created for this purpose.},
      keywords     = {Accuracy (Other) / RNA (Other) / RT-qPCR (Other) /
                      quantification (Other)},
      cin          = {BE01},
      ddc          = {610},
      cid          = {I:(DE-He78)BE01-20160331},
      pnm          = {899 - ohne Topic (POF4-899)},
      pid          = {G:(DE-HGF)POF4-899},
      typ          = {PUB:(DE-HGF)16},
      pubmed       = {pmid:36916500},
      doi          = {10.1002/1878-0261.13418},
      url          = {https://inrepo02.dkfz.de/record/274310},
}