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@ARTICLE{Vangalis:274319,
author = {V. Vangalis and E. A. Markakis and M. Knop$^*$ and A. D.
Pietro and M. A. Typas and I. A. Papaioannou},
title = {{C}omponents of {TOR} and {MAP} kinase signaling control
chemotropism and pathogenicity in the fungal pathogen
{V}erticillium dahliae.},
journal = {Microbiological research},
volume = {271},
issn = {0944-5013},
address = {Jena},
publisher = {Urban $\&$ Fischer},
reportid = {DKFZ-2023-00533},
pages = {127361},
year = {2023},
note = {DKFZ-ZMBH Alliance},
abstract = {Filamentous fungi can sense useful resources and hazards in
their environment and direct growth of their hyphae
accordingly. Chemotropism ensures access to nutrients,
contact with other individuals (e.g., for mating), and
interaction with hosts in the case of pathogens. Previous
studies have revealed a complex chemotropic sensing
landscape during host-pathogen interactions, but the
underlying molecular machinery remains poorly characterized.
Here we studied mechanisms controlling directed hyphal
growth of the important plant-pathogenic fungus Verticillium
dahliae towards different chemoattractants. We found that
the homologs of the Rag GTPase Gtr1 and the
GTPase-activating protein Tsc2, an activator and a repressor
of the TOR kinase respectively, play important roles in
hyphal chemotropism towards nutrients, plant-derived
signals, and heterologous α-pheromone of Fusarium
oxysporum. Furthermore, important roles of these regulators
were identified in fungal development and pathogenicity. We
also found that the mitogen-activated protein kinase (MAPK)
Fus3 is required for chemotropism towards nutrients, while
the G protein-coupled receptor (GPCR) Ste2 and the MAPK Slt2
control chemosensing of plant-derived signals and
α-pheromone. Our study establishes V. dahliae as a suitable
model system for the analysis of fungal chemotropism and
discovers new components of chemotropic signaling during
growth and host-pathogen interactions of V. dahliae.},
keywords = {Autophagy (Other) / Chemotropism (Other) / Environmental
sensing (Other) / Pathogenicity (Other) / Verticillium
dahliae (Other) / mTOR signaling pathway (Other)},
cin = {A260},
ddc = {570},
cid = {I:(DE-He78)A260-20160331},
pnm = {311 - Zellbiologie und Tumorbiologie (POF4-311)},
pid = {G:(DE-HGF)POF4-311},
typ = {PUB:(DE-HGF)16},
pubmed = {pmid:36921400},
doi = {10.1016/j.micres.2023.127361},
url = {https://inrepo02.dkfz.de/record/274319},
}