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@ARTICLE{Zecha:274352,
author = {J. Zecha$^*$ and F. P. Bayer and S. Wiechmann$^*$ and J.
Woortman and N. Berner$^*$ and J. Müller and A. Schneider
and K. Kramer and M. Abril-Gil and T. Hopf and L. Reichart
and L. Chen and F. M. Hansen and S. Lechner and P. Samaras
and S. Eckert$^*$ and L. Lautenbacher and M. Reinecke and F.
Hamood and P. Prokofeva and L. Vornholz and C.
Falcomatà$^*$ and M. Dorsch$^*$ and A. Schröder and A.
Venhuizen and S. Wilhelm and G. Médard and G. Stoehr and J.
Ruland$^*$ and B. Grüner$^*$ and D. K. M. Saur$^*$ and M.
Buchner and B. Ruprecht and H. Hahne and M. The and M.
Wilhelm and B. Kuster$^*$},
title = {{D}ecrypting drug actions and protein modifications by
dose- and time-resolved proteomics.},
journal = {Science},
volume = {380},
number = {6640},
issn = {0036-8075},
address = {Cambridge, Mass.},
publisher = {Moses King},
reportid = {DKFZ-2023-00554},
pages = {93-101},
year = {2023},
note = {2023 Apr 7;380(6640):93-101},
abstract = {Although most cancer drugs modulate the activities of
cellular pathways by changing post-translational
modifications (PTMs), surprisingly little is known regarding
the extent and the time- and dose-response characteristics
of drug-regulated PTMs. Here, we introduce a proteomic assay
termed decryptM that quantifies drug-PTM modulation for
thousands of PTMs in cells to shed light on target
engagement and drug mechanism of action (MoA). Examples
range from detecting DNA damage by chemotherapeutics, to
identifying drug-specific PTM signatures of kinase
inhibitors, to demonstrating that rituximab kills
CD20-positive B-cells by over-activating B cell receptor
signaling. DecryptM profiling of 31 cancer drugs in 13 cell
lines demonstrates the broad applicability of the approach.
The resulting 1.8 million dose-response curves are provided
as an interactive molecular resource in ProteomicsDB.},
cin = {MU01 / ED01},
ddc = {500},
cid = {I:(DE-He78)MU01-20160331 / I:(DE-He78)ED01-20160331},
pnm = {899 - ohne Topic (POF4-899)},
pid = {G:(DE-HGF)POF4-899},
typ = {PUB:(DE-HGF)16},
pubmed = {pmid:36926954},
doi = {10.1126/science.ade3925},
url = {https://inrepo02.dkfz.de/record/274352},
}