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@ARTICLE{Hysenaj:274363,
      author       = {L. Hysenaj and S. Little and K. Kulhanek and M. Magnen and
                      K. Bahl and O. M. Gbenedio and M. Prinz and L. Rodriguez and
                      C. Andersen and A. A. Rao and A. Shen and J.-C. Lone and L.
                      C. Lupin-Jimenez and L. R. Bonser and N. K. Serwas and E.
                      Mick and M. M. Khalid and T. Y. Taha and R. Kumar and J. Z.
                      Li and V. W. Ding and S. Matsumoto and M. Maishan and B.
                      Sreekumar and C. Simoneau and I. Nazarenko$^*$ and M. G.
                      Tomlinson and K. Khan and A. von Gottberg and A. Sigal and
                      M. R. Looney and G. K. Fragiadakis and D. M. Jablons and C.
                      R. Langelier and M. Matthay and M. Krummel and D. J. Erle
                      and A. J. Combes and A. Sil and M. Ott and J. R. Kratz and
                      J. P. Roose},
      title        = {{SARS}-{C}o{V}-2 infection of airway organoids reveals
                      conserved use of {T}etraspanin-8 by {A}ncestral, {D}elta,
                      and {O}micron variants.},
      journal      = {Stem cell reports},
      volume       = {18},
      number       = {3},
      issn         = {2213-6711},
      address      = {[New York, NY]},
      publisher    = {Elsevier},
      reportid     = {DKFZ-2023-00565},
      pages        = {636 - 653},
      year         = {2023},
      abstract     = {Ancestral SARS coronavirus-2 (SARS-CoV-2) and variants of
                      concern (VOC) caused a global pandemic with a spectrum of
                      disease severity. The mechanistic explaining variations
                      related to airway epithelium are relatively understudied.
                      Here, we biobanked airway organoids (AO) by preserving stem
                      cell function. We optimized viral infection with H1N1/PR8
                      and comprehensively characterized epithelial responses to
                      SARS-CoV-2 infection in phenotypically stable AO from 20
                      different subjects. We discovered Tetraspanin-8 (TSPAN8) as
                      a facilitator of SARS-CoV-2 infection. TSPAN8 facilitates
                      SARS-CoV-2 infection rates independently of ACE2-Spike
                      interaction. In head-to-head comparisons with Ancestral
                      SARS-CoV-2, Delta and Omicron VOC displayed lower overall
                      infection rates of AO but triggered changes in epithelial
                      response. All variants shared highest tropism for ciliated
                      and goblet cells. TSPAN8-blocking antibodies diminish
                      SARS-CoV-2 infection and may spur novel avenues for COVID-19
                      therapy.},
      keywords     = {Humans / COVID-19 / SARS-CoV-2 / Influenza A Virus, H1N1
                      Subtype / Organoids / Tetraspanins: genetics / H1N1 (Other)
                      / SARS-CoV-2 (Other) / TSPAN8 (Other) / airway organoids
                      (Other) / cell composition (Other) / single cell RNAseq
                      (Other) / spectral flow (Other) / therapeutics (Other) /
                      variants (Other) / virus (Other) / Tetraspanins (NLM
                      Chemicals) / TSPAN8 protein, human (NLM Chemicals)},
      cin          = {FR01},
      ddc          = {610},
      cid          = {I:(DE-He78)FR01-20160331},
      pnm          = {899 - ohne Topic (POF4-899)},
      pid          = {G:(DE-HGF)POF4-899},
      typ          = {PUB:(DE-HGF)16},
      pubmed       = {pmid:36827975},
      pmc          = {pmc:PMC9948283},
      doi          = {10.1016/j.stemcr.2023.01.011},
      url          = {https://inrepo02.dkfz.de/record/274363},
}