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@ARTICLE{Peng:274377,
      author       = {X. Peng and Ž. Janićijević and S. Lemm and M. Laube and
                      J. Pietzsch and M. Bachmann$^*$ and L. Baraban},
      title        = {{S}hell engineering in soft alginate-based capsules for
                      culturing liver spheroids.},
      journal      = {Biotechnology journal},
      volume       = {18},
      number       = {6},
      issn         = {1860-6768},
      address      = {Weinheim},
      publisher    = {Wiley-VCH},
      reportid     = {DKFZ-2023-00577},
      pages        = {e2200365},
      year         = {2023},
      note         = {2023 Jun;18(6):e2200365},
      abstract     = {Functional interaction between cancer cells and the
                      surrounding microenvironment is still not sufficiently
                      understood, which motivates the tremendous interest for the
                      development of numerous in vitro tumor models. Diverse
                      parameters, e.g., transport of nutrients and metabolites,
                      availability of space in the confinement, etc. make an
                      impact on the size, shape, and metabolism of the tumoroids.
                      We demonstrate the fluidics-based low-cost methodology to
                      reproducibly generate the alginate and alginate-chitosan
                      microcapsules and apply it to grow human hepatoma (HepG2)
                      spheroids of different dimensions and geometries. Focusing
                      specifically on the composition and thickness of the
                      hydrogel shell, permeability of the microcapsules was
                      selectively tuned. The diffusion of the selected benchmark
                      molecules through the shell has been systematically
                      investigated using both, experiments and simulations, which
                      is essential to ensure efficient mass transfer and/or
                      filtering of the biochemical species. Metabolic activity of
                      spheroids in microcapsules was confirmed by tracking the
                      turnover of testosterone to androstenedione with
                      chromatography studies in a metabolic assay. Depending on
                      available space, phenotypically different 3D cell assemblies
                      have been observed inside the capsules, varying in the
                      tightness of cell aggregations and their shapes.
                      Conclusively, we believe that our system with the facile
                      tuning of the shell thickness and permeability, represents a
                      promising platform for studying the formation of cancer
                      spheroids and their functional interaction with the
                      surrounding microenvironment. This article is protected by
                      copyright. All rights reserved.},
      keywords     = {alginate and alginate-chitosan microcapsules (Other) /
                      human hepatoma cell line (HepG2) (Other) / liver spheroids
                      (Other) / microfluidic droplet generation system (Other) /
                      permeability (Other)},
      cin          = {DD01},
      ddc          = {570},
      cid          = {I:(DE-He78)DD01-20160331},
      pnm          = {899 - ohne Topic (POF4-899)},
      pid          = {G:(DE-HGF)POF4-899},
      typ          = {PUB:(DE-HGF)16},
      pubmed       = {pmid:36942860},
      doi          = {10.1002/biot.202200365},
      url          = {https://inrepo02.dkfz.de/record/274377},
}