TY  - JOUR
AU  - Tredup, Claudia
AU  - Ndreshkjana, Benardina
AU  - Schneider, Natalie S
AU  - Tjaden, Amelie
AU  - Kemas, Aurino M
AU  - Youhanna, Sonia
AU  - Lauschke, Volker M
AU  - Berger, Benedict-Tilman
AU  - Krämer, Andreas
AU  - Berger, Lena M
AU  - Röhm, Sandra
AU  - Knapp, Stefan
AU  - Farin, Henner
AU  - Müller, Susanne
TI  - Deep Annotation of Donated Chemical Probes (DCP) in Organotypic Human Liver Cultures and Patient-Derived Organoids from Tumor and Normal Colorectum.
JO  - ACS chemical biology
VL  - 18
IS  - 4
SN  - 1554-8929
CY  - Washington, DC
PB  - Soc.
M1  - DKFZ-2023-00578
SP  - 822-836
PY  - 2023
N1  - 2023 Apr 21;18(4):822-836
AB  - Well-characterized small molecules are essential tools for studying the biology and therapeutic relevance of a target protein. However, many compounds reported in the literature and routinely studied in biomedical research lack the potency and selectivity required for mechanistic cellular studies on the function of a given protein. Furthermore, commercially available compounds often do not include useful tools developed by industry as part of their research and development efforts, as they frequently remain proprietary. The freely available donated chemical probe (DCP) library, fueled by generous donations of compounds from industry and academia, enables easy access to a steadily growing collection of these valuable and well-characterized tools. Here, we provide a systematic description of the current DCP library collection and their associated comprehensive characterization data, including a variety of in vitro and cellular assays. Of note, we characterized the set in relevant human primary models by employing hepatotoxicity screening in primary human liver spheroids and viability screening in patient-derived colorectal cancer organoids and matched normal-adjacent epithelium. Taken together, the DCP library represents a well-annotated, openly available collection of tool compounds for studying a wide range of targets, including kinases, G-protein-coupled receptors, and ion channels. As such, it represents a unique resource for the biomedical research community.
LB  - PUB:(DE-HGF)16
C6  - pmid:36944371
DO  - DOI:10.1021/acschembio.2c00877
UR  - https://inrepo02.dkfz.de/record/274378
ER  -