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@ARTICLE{Tredup:274378,
author = {C. Tredup and B. Ndreshkjana and N. S. Schneider and A.
Tjaden and A. M. Kemas and S. Youhanna and V. M. Lauschke
and B.-T. Berger and A. Krämer and L. M. Berger and S.
Röhm and S. Knapp and H. Farin$^*$ and S. Müller},
title = {{D}eep {A}nnotation of {D}onated {C}hemical {P}robes
({DCP}) in {O}rganotypic {H}uman {L}iver {C}ultures and
{P}atient-{D}erived {O}rganoids from {T}umor and {N}ormal
{C}olorectum.},
journal = {ACS chemical biology},
volume = {18},
number = {4},
issn = {1554-8929},
address = {Washington, DC},
publisher = {Soc.},
reportid = {DKFZ-2023-00578},
pages = {822-836},
year = {2023},
note = {2023 Apr 21;18(4):822-836},
abstract = {Well-characterized small molecules are essential tools for
studying the biology and therapeutic relevance of a target
protein. However, many compounds reported in the literature
and routinely studied in biomedical research lack the
potency and selectivity required for mechanistic cellular
studies on the function of a given protein. Furthermore,
commercially available compounds often do not include useful
tools developed by industry as part of their research and
development efforts, as they frequently remain proprietary.
The freely available donated chemical probe (DCP) library,
fueled by generous donations of compounds from industry and
academia, enables easy access to a steadily growing
collection of these valuable and well-characterized tools.
Here, we provide a systematic description of the current DCP
library collection and their associated comprehensive
characterization data, including a variety of in vitro and
cellular assays. Of note, we characterized the set in
relevant human primary models by employing hepatotoxicity
screening in primary human liver spheroids and viability
screening in patient-derived colorectal cancer organoids and
matched normal-adjacent epithelium. Taken together, the DCP
library represents a well-annotated, openly available
collection of tool compounds for studying a wide range of
targets, including kinases, G-protein-coupled receptors, and
ion channels. As such, it represents a unique resource for
the biomedical research community.},
cin = {FM01},
ddc = {540},
cid = {I:(DE-He78)FM01-20160331},
pnm = {899 - ohne Topic (POF4-899)},
pid = {G:(DE-HGF)POF4-899},
typ = {PUB:(DE-HGF)16},
pubmed = {pmid:36944371},
doi = {10.1021/acschembio.2c00877},
url = {https://inrepo02.dkfz.de/record/274378},
}