TY  - JOUR
AU  - Schallenberg, Simon
AU  - Dragomir, Mihnea-Paul
AU  - Anders, Philipp
AU  - Ebner, Benedikt
AU  - Volz, Yannic
AU  - Eismann, Lennert
AU  - Rodler, Severin
AU  - Casuscelli, Jozefina
AU  - Buchner, Alexander
AU  - Klauschen, Frederick
AU  - Stief, Christian
AU  - Horst, David
AU  - Schulz, Gerald Bastian
TI  - Intratumoral Heterogeneity of Molecular Subtypes in Muscle-invasive Bladder Cancer-An Extensive Multiregional Immunohistochemical Analysis.
JO  - European urology focus
VL  - 9
IS  - 5
SN  - 2405-4569
CY  - Amsterdam
PB  - Elsevier
M1  - DKFZ-2023-00789
SP  - 788-798
PY  - 2023
N1  - 2023 Sep;9(5):788-798
AB  - Molecular bladder cancer (BC) subtypes define distinct biological entities and were shown to predict treatment response in neoadjuvant and adjuvant settings. The extent of intratumoral heterogeneity (ITH) might affect subtyping of individual patients.To comprehensively assess the ITH of molecular subtypes in a cohort of muscle-invasive BC.A total of 251 patients undergoing radical cystectomy were screened. Three cores of the tumor center (TC) and three cores of the invasive tumor front (TF) of each patient were assembled in a tissue microarray. Molecular subtypes were determined employing 12 pre-evaluated immunohistochemical markers (FGFR3, CCND1, RB1, CDKN2A, KRT5, KRT14, FOXA1, GATA3, TUBB2B, EPCAM, CDH1, and vimentin). A total of 18 072 spots were evaluated, of which 15 002 spots were assessed based on intensity, distribution, or combination.Allocation to one of five different molecular subtypes-urothelial like, genomically unstable, small-cell/neuroendocrine like, basal/squamous cell carcinoma like, and mesenchymal like-was conducted for each patient for the complete tumor, individual cores, TF, and TC separately. The primary objective was to assess the ITH between the TF and TC (n = 208 patients). The secondary objective was the evaluation of multiregion ITH (n = 191 patients). An analysis of the composition of ITH cases, association with clinicopathological parameters, and prognosis was conducted.ITH between the TF and TC was seen in 12.5
KW  - Bladder cancer (Other)
KW  - Immunohistochemistry (Other)
KW  - Intratumoral heterogeneity (Other)
KW  - Molecular subtyping (Other)
KW  - Urothelial carcinoma (Other)
LB  - PUB:(DE-HGF)16
C6  - pmid:37076398
DO  - DOI:10.1016/j.euf.2023.03.012
UR  - https://inrepo02.dkfz.de/record/275482
ER  -