TY  - JOUR
AU  - Kerkhoff, Maximilian
AU  - Grunewald, Susanne
AU  - Schaefer, Christiane
AU  - Zöllner, Stefan K
AU  - Plaumann, Pauline
AU  - Busch, Maike
AU  - Dünker, Nicole
AU  - Ketzer, Julia
AU  - Kersting, Josephine
AU  - Bauer, Sebastian
AU  - Hardes, Jendrik
AU  - Streitbürger, Arne
AU  - Dirksen, Uta
AU  - Hartmann, Wolfgang
AU  - Guder, Wiebke K
TI  - Evaluation of the Effect of Photodynamic Therapy on CAM-Grown Sarcomas.
JO  - Bioengineering
VL  - 10
IS  - 4
SN  - 2306-5354
CY  - Basel
PB  - MDPI
M1  - DKFZ-2023-00843
SP  - 464
PY  - 2023
AB  - Resection margin adequacy plays a critical role in the local control of sarcomas. Fluorescence-guided surgery has increased complete resection rates and local recurrence-free survival in several oncological disciplines. The purpose of this study was to determine whether sarcomas exhibit sufficient tumor fluorescence (photodynamic diagnosis (PDD)) after administration of 5-aminolevulinic acid (5-ALA) and whether photodynamic therapy (PDT) has an impact on tumor vitality in vivo. Sixteen primary cell cultures were derived from patient samples of 12 different sarcoma subtypes and transplanted onto the chorio-allantoic membrane (CAM) of chick embryos to generate 3-dimensional cell-derived xenografts (CDXs). After treatment with 5-ALA, the CDXs were incubated for another 4 h. Subsequently accumulated protoporphyrin IX (PPIX) was excited by blue light and the intensity of tumor fluorescence was analyzed. A subset of CDXs was exposed to red light and morphological changes of both CAMs and tumors were documented. Twenty-four hours after PDT, the tumors were excised and examined histologically. High rates of cell-derived engraftments on the CAM were achieved in all sarcoma subtypes and an intense PPIX fluorescence was observed. PDT of CDXs resulted in a disruption of tumor-feeding vessels and 52.4
KW  - 5-ALA (Other)
KW  - 5-aminolevulinic acid (Other)
KW  - CAM (Other)
KW  - cell-derived xenografts (Other)
KW  - chorion-allantois membrane model (Other)
KW  - photodynamic diagnostics (Other)
KW  - photodynamic therapy (Other)
KW  - sarcoma (Other)
KW  - tumor fluorescence (Other)
LB  - PUB:(DE-HGF)16
C6  - pmid:37106651
DO  - DOI:10.3390/bioengineering10040464
UR  - https://inrepo02.dkfz.de/record/275655
ER  -