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@ARTICLE{Kerkhoff:275655,
      author       = {M. Kerkhoff$^*$ and S. Grunewald$^*$ and C. Schaefer$^*$
                      and S. K. Zöllner$^*$ and P. Plaumann$^*$ and M. Busch$^*$
                      and N. Dünker$^*$ and J. Ketzer$^*$ and J. Kersting$^*$ and
                      S. Bauer$^*$ and J. Hardes$^*$ and A. Streitbürger$^*$ and
                      U. Dirksen$^*$ and W. Hartmann and W. K. Guder$^*$},
      title        = {{E}valuation of the {E}ffect of {P}hotodynamic {T}herapy on
                      {CAM}-{G}rown {S}arcomas.},
      journal      = {Bioengineering},
      volume       = {10},
      number       = {4},
      issn         = {2306-5354},
      address      = {Basel},
      publisher    = {MDPI},
      reportid     = {DKFZ-2023-00843},
      pages        = {464},
      year         = {2023},
      abstract     = {Resection margin adequacy plays a critical role in the
                      local control of sarcomas. Fluorescence-guided surgery has
                      increased complete resection rates and local recurrence-free
                      survival in several oncological disciplines. The purpose of
                      this study was to determine whether sarcomas exhibit
                      sufficient tumor fluorescence (photodynamic diagnosis (PDD))
                      after administration of 5-aminolevulinic acid (5-ALA) and
                      whether photodynamic therapy (PDT) has an impact on tumor
                      vitality in vivo. Sixteen primary cell cultures were derived
                      from patient samples of 12 different sarcoma subtypes and
                      transplanted onto the chorio-allantoic membrane (CAM) of
                      chick embryos to generate 3-dimensional cell-derived
                      xenografts (CDXs). After treatment with 5-ALA, the CDXs were
                      incubated for another 4 h. Subsequently accumulated
                      protoporphyrin IX (PPIX) was excited by blue light and the
                      intensity of tumor fluorescence was analyzed. A subset of
                      CDXs was exposed to red light and morphological changes of
                      both CAMs and tumors were documented. Twenty-four hours
                      after PDT, the tumors were excised and examined
                      histologically. High rates of cell-derived engraftments on
                      the CAM were achieved in all sarcoma subtypes and an intense
                      PPIX fluorescence was observed. PDT of CDXs resulted in a
                      disruption of tumor-feeding vessels and $52.4\%$ of CDXs
                      presented as regressive after PDT treatment, whereas control
                      CDXs remained vital in all cases. Therefore, 5-ALA mediated
                      PDD and PDT appear to be promising tools in defining sarcoma
                      resection margins (PDD) and adjuvant treatment of the tumor
                      bed (PDT).},
      keywords     = {5-ALA (Other) / 5-aminolevulinic acid (Other) / CAM (Other)
                      / cell-derived xenografts (Other) / chorion-allantois
                      membrane model (Other) / photodynamic diagnostics (Other) /
                      photodynamic therapy (Other) / sarcoma (Other) / tumor
                      fluorescence (Other)},
      cin          = {ED01},
      ddc          = {570},
      cid          = {I:(DE-He78)ED01-20160331},
      pnm          = {899 - ohne Topic (POF4-899)},
      pid          = {G:(DE-HGF)POF4-899},
      typ          = {PUB:(DE-HGF)16},
      pubmed       = {pmid:37106651},
      doi          = {10.3390/bioengineering10040464},
      url          = {https://inrepo02.dkfz.de/record/275655},
}