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000275770 1001_ $$0P:(DE-He78)17064a887f14c1cee3ae16af3cf73314$$aPervaiz, Asim$$b0$$eFirst author
000275770 245__ $$aAnticancer genes (NOXA, PAR-4, TRAIL) are de-regulated in breast cancer patients and can be targeted by using a ribosomal inactivating plant protein (riproximin).
000275770 260__ $$aDordrecht [u.a.]$$bSpringer Science + Business Media B.V$$c2023
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000275770 520__ $$aAnticancer genes are an endogenous defense against transformed cells as they impose antineoplastic effects upon ectopic expression. Profiling the expression of these genes is fundamental for exploring their prognostic and therapeutic relevance in cancers. Natural compounds can upregulate anticancer genes in malignant cells and thus be useful for therapeutic purposes. In this study, we identified the expression levels of anticancer genes in breast cancer clinical isolates. In addition, the purified and sequenced plant protein (riproximin) was evaluated for its potential to induce anticancer genes in two breast cancer cell lines.Expression profiles of three anticancer genes (NOXA, PAR-4, TRAIL) were identified by immunohistochemistry in 45 breast cancer clinical isolates. Breast cancer cells were exposed to riproximin and expression of the anticancer genes was determined by microarray, real-time PCR and western blot methodologies. Lastly, a bioinformatic approach was adopted to highlight the molecular/functional significance of the anticancer genes.NOXA expression was evenly de-regulated among the clinical isolates, while PAR-4 was significantly down-regulated in majority of the breast cancer tissues. In contrast, TRAIL expression was increased in most of the clinical samples. Expression levels of the anticancer genes followed a distinct trend in accordance with the disease severity. Riproximin showed a substantial potential of inducing expression of the anticancer genes in breast cancer cells at transcriptomic and protein levels. The bioinformatic approach revealed involvement of anticancer genes in multiple cellular functions and signaling cascades.Anticancer genes were de-regulated and showed discrete expression patterns in breast cancer patient samples. Riproximin effectively induced the expression of selected anticancer genes in breast cancer cells.
000275770 536__ $$0G:(DE-HGF)POF4-311$$a311 - Zellbiologie und Tumorbiologie (POF4-311)$$cPOF4-311$$fPOF IV$$x0
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000275770 650_7 $$2Other$$aAnticancer genes
000275770 650_7 $$2Other$$aBreast cancer
000275770 650_7 $$2Other$$aPlant protein
000275770 650_7 $$2Other$$aRiproximin
000275770 7001_ $$aNaseem, Nadia$$b1
000275770 7001_ $$aSaleem, Talha$$b2
000275770 7001_ $$aRaza, Syed Mohsin$$b3
000275770 7001_ $$aShaukat, Iqra$$b4
000275770 7001_ $$aKanwal, Kinzah$$b5
000275770 7001_ $$aSajjad, Osheen$$b6
000275770 7001_ $$aIqbal, Sana$$b7
000275770 7001_ $$aShams, Faiza$$b8
000275770 7001_ $$aIjaz, Bushra$$b9
000275770 7001_ $$0P:(DE-He78)7e60033e3eaaebb9ba30c905ade4a676$$aBerger, Martin$$b10$$eLast author
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