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@ARTICLE{Pervaiz:275770,
      author       = {A. Pervaiz$^*$ and N. Naseem and T. Saleem and S. M. Raza
                      and I. Shaukat and K. Kanwal and O. Sajjad and S. Iqbal and
                      F. Shams and B. Ijaz and M. Berger$^*$},
      title        = {{A}nticancer genes ({NOXA}, {PAR}-4, {TRAIL}) are
                      de-regulated in breast cancer patients and can be targeted
                      by using a ribosomal inactivating plant protein
                      (riproximin).},
      journal      = {Molecular biology reports},
      volume       = {50},
      number       = {6},
      issn         = {0301-4851},
      address      = {Dordrecht [u.a.]},
      publisher    = {Springer Science + Business Media B.V},
      reportid     = {DKFZ-2023-00869},
      pages        = {5209-5221},
      year         = {2023},
      note         = {2023 Jun;50(6):5209-5221 / #EA:G401#LA:G401#},
      abstract     = {Anticancer genes are an endogenous defense against
                      transformed cells as they impose antineoplastic effects upon
                      ectopic expression. Profiling the expression of these genes
                      is fundamental for exploring their prognostic and
                      therapeutic relevance in cancers. Natural compounds can
                      upregulate anticancer genes in malignant cells and thus be
                      useful for therapeutic purposes. In this study, we
                      identified the expression levels of anticancer genes in
                      breast cancer clinical isolates. In addition, the purified
                      and sequenced plant protein (riproximin) was evaluated for
                      its potential to induce anticancer genes in two breast
                      cancer cell lines.Expression profiles of three anticancer
                      genes (NOXA, PAR-4, TRAIL) were identified by
                      immunohistochemistry in 45 breast cancer clinical isolates.
                      Breast cancer cells were exposed to riproximin and
                      expression of the anticancer genes was determined by
                      microarray, real-time PCR and western blot methodologies.
                      Lastly, a bioinformatic approach was adopted to highlight
                      the molecular/functional significance of the anticancer
                      genes.NOXA expression was evenly de-regulated among the
                      clinical isolates, while PAR-4 was significantly
                      down-regulated in majority of the breast cancer tissues. In
                      contrast, TRAIL expression was increased in most of the
                      clinical samples. Expression levels of the anticancer genes
                      followed a distinct trend in accordance with the disease
                      severity. Riproximin showed a substantial potential of
                      inducing expression of the anticancer genes in breast cancer
                      cells at transcriptomic and protein levels. The
                      bioinformatic approach revealed involvement of anticancer
                      genes in multiple cellular functions and signaling
                      cascades.Anticancer genes were de-regulated and showed
                      discrete expression patterns in breast cancer patient
                      samples. Riproximin effectively induced the expression of
                      selected anticancer genes in breast cancer cells.},
      keywords     = {Anticancer genes (Other) / Breast cancer (Other) / Plant
                      protein (Other) / Riproximin (Other)},
      cin          = {G401},
      ddc          = {570},
      cid          = {I:(DE-He78)G401-20160331},
      pnm          = {311 - Zellbiologie und Tumorbiologie (POF4-311)},
      pid          = {G:(DE-HGF)POF4-311},
      typ          = {PUB:(DE-HGF)16},
      pubmed       = {pmid:37127809},
      doi          = {10.1007/s11033-023-08477-3},
      url          = {https://inrepo02.dkfz.de/record/275770},
}