TY  - JOUR
AU  - Chamorro González, Rocío
AU  - Conrad, Thomas
AU  - Stöber, Maja C
AU  - Xu, Robin
AU  - Giurgiu, Mădălina
AU  - Rodriguez-Fos, Elias
AU  - Kasack, Katharina
AU  - Brückner, Lotte
AU  - van Leen, Eric
AU  - Helmsauer, Konstantin
AU  - Dorado Garcia, Heathcliff
AU  - Stefanova, Maria E
AU  - Hung, King L
AU  - Bei, Yi
AU  - Schmelz, Karin
AU  - Lodrini, Marco
AU  - Mundlos, Stefan
AU  - Chang, Howard Y
AU  - Deubzer, Hedwig E
AU  - Sauer, Sascha
AU  - Eggert, Angelika
AU  - Schulte, Johannes
AU  - Schwarz, Roland F
AU  - Haase, Kerstin
AU  - Koche, Richard P
AU  - Henssen, Anton G
TI  - Parallel sequencing of extrachromosomal circular DNAs and transcriptomes in single cancer cells.
JO  - Nature genetics
VL  - 55
IS  - 5
SN  - 1061-4036
CY  - London
PB  - Macmillan Publishers Limited, part of Springer Nature
M1  - DKFZ-2023-00881
SP  - 880-890
PY  - 2023
N1  - 2023 May;55(5):880-890
AB  - Extrachromosomal DNAs (ecDNAs) are common in cancer, but many questions about their origin, structural dynamics and impact on intratumor heterogeneity are still unresolved. Here we describe single-cell extrachromosomal circular DNA and transcriptome sequencing (scEC</td><td width="150">
AB  - T-seq), a method for parallel sequencing of circular DNAs and full-length mRNA from single cells. By applying scEC</td><td width="150">
AB  - T-seq to cancer cells, we describe intercellular differences in ecDNA content while investigating their structural heterogeneity and transcriptional impact. Oncogene-containing ecDNAs were clonally present in cancer cells and drove intercellular oncogene expression differences. In contrast, other small circular DNAs were exclusive to individual cells, indicating differences in their selection and propagation. Intercellular differences in ecDNA structure pointed to circular recombination as a mechanism of ecDNA evolution. These results demonstrate scEC</td><td width="150">
AB  - T-seq as an approach to systematically characterize both small and large circular DNA in cancer cells, which will facilitate the analysis of these DNA elements in cancer and beyond.
LB  - PUB:(DE-HGF)16
C6  - pmid:37142849
DO  - DOI:10.1038/s41588-023-01386-y
UR  - https://inrepo02.dkfz.de/record/275793
ER  -